Razin S, Tully J G
J Bacteriol. 1970 May;102(2):306-10. doi: 10.1128/jb.102.2.306-310.1970.
Cholesterol requirement for growth of mycoplasmas was tested in a serum-free medium supplemented with albumin, l-arginine, palmitic acid, and various concentrations of cholesterol dissolved in Tween 80. In cases in which Tween 80 was shown to inhibit growth, the test medium was supplemented with cholesterol dissolved in ethanol. Of the 31 species examined, all but Mycoplasma laidlawii, M. granularum, and Mycoplasma species strain S-743 exhibited a growth response to cholesterol. No requirement for cholesterol could be shown with the stable L-phase variants of Streptobacillus moniliformis and Proteus species. The results provide experimental support for the view that the large majority of the established Mycoplasma species require cholesterol for growth.
在一种无血清培养基中测试了支原体生长对胆固醇的需求，该培养基添加了白蛋白、L-精氨酸、棕榈酸以及溶解于吐温80中的不同浓度胆固醇。在吐温80显示出抑制生长的情况下，测试培养基添加了溶解于乙醇的胆固醇。在所检测的31个物种中，除莱氏无胆甾原体、颗粒支原体和支原体物种菌株S-743外，所有物种均对胆固醇表现出生长反应。念珠状链杆菌和变形杆菌属的稳定L型变体未显示出对胆固醇的需求。这些结果为以下观点提供了实验支持，即绝大多数已确定的支原体物种生长需要胆固醇。
