Asano Tetsuichi, Kaneko Eiji, Shinozaki Shohei, Imai Yutaka, Shibayama Masaharu, Chiba Tsuyoshi, Ai Masumi, Kawakami Akio, Asaoka Hiroshi, Nakayama Toru, Mano Yoshihiro, Shimokado Kentaro
Geriatrics and Vascular Medicine, Tokyo Medical and Dental University Graduate School, Tokyo, Japan.
Circ J. 2007 Mar;71(3):405-11. doi: 10.1253/circj.71.405.
It is not clear how hyperbaric oxygen therapy (HBO) affects ischemia-induced pathophysiological responses such as angiogenesis and skeletal muscle regeneration. In the present study the effects of HBO on the functional and morphological recovery of ischemic hind limbs, blood perfusion and the local production of angiogenic growth factors were studied in a mouse model.
Mice were placed in pure oxygen under 3 atm for 1 h/day for 14 days after the removal of a segment of the left femoral artery. HBO-treated mice showed better functional recovery and greater blood flow in the ischemic hind limb than untreated mice. Histological examination revealed unatrophied muscle fibers with islands of small regenerating muscle cells only in HBO-treated mice. Regeneration of muscle was confirmed by the increase in myf5 mRNA. The amount of mRNA for vascular endothelial growth factor (VEGF), hepatocyte growth factor (HGF) and basic fibroblast growth factor (bFGF) was slightly increased in the ischemic hind limbs. HBO eliminated the increase in VEGF mRNA. In contrast, the amount of mRNA for bFGF and HGF was further increased by HBO treatment. HBO transiently increased early growth response protein 1 (Egr-1) in the ischemic hind limbs.
HBO accelerates the recovery of ischemic hind limbs by increasing the production of bFGF and HGF and by promoting muscle regeneration in mice.
尚不清楚高压氧疗法（HBO）如何影响缺血诱导的病理生理反应，如血管生成和骨骼肌再生。在本研究中，在小鼠模型中研究了HBO对缺血后肢功能和形态恢复、血液灌注以及血管生成生长因子局部产生的影响。
在切除一段左股动脉后，将小鼠置于3个大气压的纯氧环境中，每天1小时，持续14天。与未接受治疗的小鼠相比，接受HBO治疗的小鼠缺血后肢功能恢复更好，血流更大。组织学检查显示，仅在接受HBO治疗的小鼠中存在未萎缩的肌纤维以及小的再生肌细胞岛。通过myf5 mRNA的增加证实了肌肉再生。缺血后肢中血管内皮生长因子（VEGF）、肝细胞生长因子（HGF）和碱性成纤维细胞生长因子（bFGF）的mRNA量略有增加。HBO消除了VEGF mRNA的增加。相反，HBO治疗进一步增加了bFGF和HGF的mRNA量。HBO使缺血后肢中的早期生长反应蛋白1（Egr-1）短暂增加。
HBO通过增加bFGF和HGF的产生以及促进小鼠肌肉再生来加速缺血后肢的恢复。
