Gautier Anais, Graff Emily C, Bacek Lenore, Fish Eric J, White Amelia, Palmer Lee, Kuo Kendon
Department of Emergency and Critical Care, Auburn University Veterinary Teaching Hospital, Auburn, AL, United States.
Department of Pathobiology, Auburn University Veterinary Teaching Hospital, Auburn, AL, United States.
Front Vet Sci. 2020 Jan 15;6:506. doi: 10.3389/fvets.2019.00506. eCollection 2019.
Hyperbaric oxygen therapy (HBOT) involves breathing 100% oxygen in a specialized compression chamber leading to hyperoxia. This treatment modality is associated with anti-inflammatory, antioxidant, and healing properties in people and laboratory animals. However, there are relatively few reports that evaluate the effects of HBOT in companion animals. The goal of this study was to investigate the physiological effects of HBOT on surgically induced systemic inflammation and oxidation in dogs. Twelve healthy female beagle dogs were spayed and randomized into control and HBOT groups ( = 6). Both groups received conventional post-ovariohysterectomy therapy, and the HBOT group received two hyperbaric treatments at 2.0 atmosphere of absolute pressure and 100% oxygen for 35 min, 6 and 18 h after surgery. Blood samples were collected 3 h prior to ovariohysterectomy, 6, 18, and 30 h after surgery, prior to HBOT when applicable. Inflammatory biomarkers, including C-reactive protein, circulating cytokines, and changes in iron homeostasis were evaluated at each time point to determine the effects of surgery and HBOT on inflammation. Similarly, serum total oxidant status and total antioxidant status were measured to assess the oxidative stress. Pain and incision scores were recorded and compared between groups. Following ovariohysterectomy, all dogs had significantly increased serum concentrations of C-reactive protein, KC-like, IL-6, and increased unsaturated iron-binding capacity compared to their pre-surgical values ( < 0.02), while serum iron, total iron-binding capacity and transferrin saturation were significantly decreased after surgery ( < 0.02). There was no significant difference between the control group and the HBOT group for any of the variables. There were no overt adverse effects in the HBOT group. This is the first prospective randomized controlled study to investigate the effects of HBOT on surgically induced systemic inflammation in dogs. While elective ovariohysterectomy resulted in mild inflammation, the described HBOT protocol portrayed no outward adverse effect and did not induce any detectable pro-inflammatory, anti-inflammatory, or antioxidant effects. Additional investigation is required to identify objective markers to quantify the response to HBOT and determine its role as an adjunctive therapy in dogs with more severe, complicated or chronic diseases.
高压氧疗法（HBOT）是指在专门的加压舱内呼吸100%的氧气，从而导致高氧状态。这种治疗方式在人和实验动物中具有抗炎、抗氧化和促进愈合的特性。然而，评估HBOT对伴侣动物影响的报告相对较少。本研究的目的是调查HBOT对手术诱导的犬全身炎症和氧化的生理影响。将12只健康的雌性比格犬进行卵巢子宫切除术，并随机分为对照组和HBOT组（每组n = 6）。两组均接受常规的卵巢子宫切除术后治疗，HBOT组在术后6小时和18小时接受两次高压治疗，绝对压力为2.0个大气压，吸入100%氧气，持续35分钟。在卵巢子宫切除术术前3小时、术后6小时、18小时和30小时（如适用，在HBOT治疗前）采集血样。在每个时间点评估炎症生物标志物，包括C反应蛋白、循环细胞因子以及铁稳态的变化，以确定手术和HBOT对炎症的影响。同样，测量血清总氧化剂状态和总抗氧化剂状态以评估氧化应激。记录并比较两组之间的疼痛和切口评分。卵巢子宫切除术后，与术前值相比，所有犬的血清C反应蛋白、KC样、白细胞介素-6浓度均显著升高，不饱和铁结合能力增加（P < 0.02），而术后血清铁、总铁结合能力和转铁蛋白饱和度显著降低（P < 0.02）。对照组和HBOT组在任何变量上均无显著差异。HBOT组没有明显的不良反应。这是第一项前瞻性随机对照研究，旨在调查HBOT对手术诱导的犬全身炎症的影响。虽然择期卵巢子宫切除术导致了轻度炎症，但所描述的HBOT方案没有明显的不良反应，也没有诱导任何可检测到的促炎、抗炎或抗氧化作用。需要进一步研究以确定客观标志物，以量化对HBOT的反应，并确定其在患有更严重、复杂或慢性疾病的犬中作为辅助治疗的作用。
