用于角捕获的纳米制造石英圆柱体：DNA超螺旋扭矩检测。

Nanofabricated quartz cylinders for angular trapping: DNA supercoiling torque detection.

作者信息

Deufel Christopher, Forth Scott, Simmons Chad R, Dejgosha Siavash, Wang Michelle D

机构信息

Department of Physics, Laboratory of Atomic and Solid State Physics, Cornell University, Ithaca, New York 14853, USA.

出版信息

Nat Methods. 2007 Mar;4(3):223-5. doi: 10.1038/nmeth1013. Epub 2007 Feb 25.

DOI:10.1038/nmeth1013

PMID:17322891

Abstract

We designed and created nanofabricated quartz cylinders well suited for torque application and detection in an angular optical trap. We made the cylinder axis perpendicular to the extraordinary axis of the quartz crystal and chemically functionalized only one end of each cylinder for attachment to a DNA molecule. We directly measured the torque on a single DNA molecule as it underwent a phase transition from B-form to supercoiled P-form.

摘要

我们设计并制造了纳米加工的石英圆柱体，非常适合在角向光阱中施加和检测扭矩。我们使圆柱体的轴垂直于石英晶体的非常光轴，并仅对每个圆柱体的一端进行化学功能化处理，以便连接到DNA分子上。我们直接测量了单个DNA分子从B型转变为超螺旋P型时所承受的扭矩。