Wang Leyu, Li Yadong
Department of Chemistry, Tsinghua University, Beijing, 100084, P.R. China.
Chemistry. 2007;13(15):4203-7. doi: 10.1002/chem.200700005.
By using a facile, wet-chemical approach, luminescent LaF3:Ce3+/Tb3+ single-crystal nanoparticles were prepared from nitrate and sodium fluoride precursors in a mixture of ethanol and ethylene glycol. These nanoparticles were functionalized with glucose. A novel fluorescence resonance energy transfer method for nonenzymatic glucose determination has been developed by using these glucose-modified nanocrystals. Under the chosen conditions, concentrations of glucose between 0.5 and 25.0 mmol L-1 in aqueous solutions were successfully determined. Owing to their high luminescence and good dispersibility in water, these nanocrystals are also potential fluorescent biolabels for other biological and clinical applications, such as in fluorescence imaging and for immunoassays.
通过一种简便的湿化学方法，以硝酸盐和氟化钠为前驱体，在乙醇和乙二醇的混合溶液中制备了发光的LaF3:Ce3+/Tb3+单晶纳米颗粒。这些纳米颗粒用葡萄糖进行了功能化修饰。利用这些葡萄糖修饰的纳米晶体，开发了一种用于非酶法测定葡萄糖的新型荧光共振能量转移方法。在选定的条件下，成功测定了水溶液中葡萄糖浓度在0.5至25.0 mmol L-1之间的含量。由于这些纳米晶体具有高发光性和在水中良好的分散性，它们也是用于其他生物和临床应用（如荧光成像和免疫测定）的潜在荧光生物标记物。
