Norikuni Masahiro, Hori Yumiko, Numata Munenori, Matsusaki Michiya, Kida Toshiyuki, Fukuhara Gaku
Department of Applied Chemistry, Osaka University, 2-1 Yamada-oka, Suita 565-0871, Japan.
Department of Chemistry, Tokyo Institute of Technology, 2-12-1 Ookayama, Meguro-ku, Tokyo 152-8551, Japan.
ACS Omega. 2024 May 13;9(20):22345-22351. doi: 10.1021/acsomega.4c01786. eCollection 2024 May 21.
The ability to sense saccharides in aqueous media has attracted much attention in multidisciplinary sciences because the detection of ultrahigh concentrations of sugar chains associated with serious diseases could lead to further health promotion. However, there are notable challenges. In this study, a rhodamine-modified Curdlan () chemosensor was synthesized that exhibited distinctive fluorescence "turn-on" responses. exhibited simultaneous sensitive and selective sensing of clinically useful acarbose with a good limit of detection (5 μM) from among those of the saccharides examined. The (chir)optical properties of were elucidated using UV/vis, fluorescence, excitation, and circular dichroism spectroscopies; lifetime measurements and morphological studies using atomic force and confocal laser scanning microscopy and dynamic light scattering techniques revealed that the fluorescence "turn-on" behavior originates from globule-to-coaggregation conversion upon insertion of the oligosaccharides in the dynamic Cur backbone.
在多学科领域中，在水性介质中感知糖类的能力备受关注，因为检测与严重疾病相关的超高浓度糖链可能有助于进一步促进健康。然而，存在显著挑战。在本研究中，合成了一种罗丹明修饰的凝胶多糖（）化学传感器，其表现出独特的荧光“开启”响应。在所检测的糖类中，该传感器对临床上有用的阿卡波糖具有同时灵敏且选择性的传感能力，检测限良好（5 μM）。利用紫外可见光谱、荧光光谱、激发光谱和圆二色光谱对该传感器的（手性）光学性质进行了阐明；通过原子力显微镜、共聚焦激光扫描显微镜和动态光散射技术进行的寿命测量和形态学研究表明，荧光“开启”行为源于寡糖插入动态凝胶多糖主链时从球状到共聚集的转变。
