用于研究所有16种血凝素亚型流感病毒聚合酶亚基基因序列的可靠通用逆转录聚合酶链反应检测方法。
Reliable universal RT-PCR assays for studying influenza polymerase subunit gene sequences from all 16 haemagglutinin subtypes.
作者信息
Li Olive T W, Barr Ian, Leung Connie Y H, Chen Honglin, Guan Yi, Peiris J S Malik, Poon Leo L M
机构信息
State Key Laboratory of Emerging Infectious Diseases, Department of Microbiology, University of Hong Kong, Queen Mary Hospital, Pokfulam, Hong Kong SAR, China.
出版信息
J Virol Methods. 2007 Jun;142(1-2):218-22. doi: 10.1016/j.jviromet.2007.01.015. Epub 2007 Feb 26.
Realizable one-step RT-PCR assays specific for influenza PB2, PB1 and PA segments are described in this report. The designs of the consensus primers were based on more than five thousands polymerase genes derived from avian or mammalian viral strains. All the viral RNA tested in this study could be consistently amplified by the assays. The reaction products were specific and could be used for direct DNA sequencing. These assays might be useful tools to study the sequences of these genes.
本报告描述了针对流感病毒PB2、PB1和PA基因片段的可实现一步法逆转录聚合酶链反应（RT-PCR）检测方法。共有引物的设计基于五千多个源自禽类或哺乳动物病毒株的聚合酶基因。本研究中检测的所有病毒RNA均可通过该检测方法持续扩增。反应产物具有特异性，可用于直接DNA测序。这些检测方法可能是研究这些基因序列的有用工具。
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