Cambrea Lee R, Hovis Jennifer S
Department of Chemistry, Purdue University, West Lafayette, Indiana 47907, USA.
Biophys J. 2007 May 15;92(10):3587-94. doi: 10.1529/biophysj.106.101139. Epub 2007 Feb 26.
The creation of three-dimensional structures in supported lipid bilayers has been examined. In bilayers, shape transformations can be triggered by adjusting a variety of parameters. Here, it is shown that bilayers composed of phosphatidylcholine and phosphatidic acid can be induced to reversibly form cap structures when exposed to an asymmetry in ionic strength. The structures that form depend on the asymmetry in the ionic strength and the amount of anionic lipid. Other factors that may be of importance in the creation of the structures, expansion forces, osmotic forces, and the bilayer-support interaction are discussed. The cap structures have the potential to be of considerable utility in examining the effect that curvature has on membrane processes.
已对支撑脂质双分子层中三维结构的形成进行了研究。在双分子层中,通过调整各种参数可以触发形状转变。在此表明,由磷脂酰胆碱和磷脂酸组成的双分子层在暴露于离子强度不对称时可被诱导可逆地形成帽状结构。形成的结构取决于离子强度的不对称性和阴离子脂质的量。还讨论了在结构形成中可能重要的其他因素,如膨胀力、渗透力和双分子层与支撑物的相互作用。帽状结构在研究曲率对膜过程的影响方面具有相当大的实用潜力。