Hossain Hamid, Landgraf Volker, Weiss Reinhard, Mann Marion, Hayatpour Jaleh, Chakraborty Trinad, Mayser Peter
Institute of Medical Microbiology, University of Giessen, Giessen, Germany.
Med Mycol. 2007 Feb;45(1):41-9. doi: 10.1080/13693780601003827.
The aim of the study was the characterization of Malassezia pachydermatis and its pigment-producing subgroup using biochemical tests and RAPD. It was of interest to determine whether particular RAPD patterns could be used to indicate pigment production, as well as a close genetic relatedness to Malassezia furfur. Therefore, 210 strains of M. pachydermatis were examined for morphology, catalase and ss-glucosidase activity, lipid and carbohydrate assimilation and the tryptophan-dependent synthesis of pigments. Of these, 114 strains were subjected to RAPD analyses. A multivariate logistic regression model was applied to classify M. pachydermatis isolates regarding their pigment production by using genetic and biological parameters. Biological and RAPD findings showed a high biological and genetic diversity within the species M. pachydermatis and within pigment producers. RAPD analysis revealed 28 genotypes within 114 strains tested. Pigment producing strains could not be assigned to a common RAPD profile, but a genetic relatedness of pigment-producing M. pachydermatis with M. furfur can be assumed. A particular RAPD pattern allowed statistically significant probability of pigment production (P<0.001) and might be used as a tool to rapidly detect pigment producing M. pachydermatis, e.g. in Malassezia-associated pityriasis versicolor. The reported method is useful for identification of pigment producing M. pachydermatis isolates and has advantages over established tests.
本研究的目的是利用生化试验和随机扩增多态性DNA（RAPD）对厚皮马拉色菌及其色素产生亚群进行特征描述。确定特定的RAPD模式是否可用于指示色素产生以及与糠秕马拉色菌的密切遗传相关性很有意义。因此，对210株厚皮马拉色菌进行了形态学、过氧化氢酶和β-葡萄糖苷酶活性、脂质和碳水化合物同化以及色氨酸依赖性色素合成的检测。其中，114株进行了RAPD分析。应用多元逻辑回归模型，通过遗传和生物学参数对厚皮马拉色菌分离株的色素产生情况进行分类。生物学和RAPD结果显示，厚皮马拉色菌物种内部以及色素产生菌内部存在高度的生物学和遗传多样性。RAPD分析在114株测试菌株中揭示了28种基因型。色素产生菌株不能归为一种常见的RAPD图谱，但可以假定产生色素的厚皮马拉色菌与糠秕马拉色菌存在遗传相关性。一种特定的RAPD模式具有色素产生的统计学显著概率（P<0.001），可作为一种工具用于快速检测产生色素的厚皮马拉色菌，例如在马拉色菌相关的花斑糠疹中。所报道的方法对于鉴定产生色素的厚皮马拉色菌分离株很有用，并且比现有检测方法具有优势。
