Sakai Akiyoshi, Akifusa Sumio, Itano Naoki, Kimata Koji, Kawamura Taro, Koseki Takeyoshi, Takehara Tadamichi, Nishihara Tatsuji
Division of Community Oral Health Science, Department of Health Promotion, Kyushu Dental College, Kitakyushu, Japan.
Med Mycol. 2007 Feb;45(1):73-9. doi: 10.1080/13693780601039607.
Candida albicans is both a commensal and a pathogen in the oral mucosa. Previous studies have indicated that epithelial cell-associated carbohydrate moiety can inhibit C. albicans growth. In the present study, the mechanisms by which epithelial cells inhibit Candida growth were studied by examining the effect of hyaluronan (HA). A coculture of C. albicans and KB cells or COS-7 cells inhibited in vitro growth of the fungus by 50-87% at an effector-to-target (E:T) ratio of 80:1. Removing extracellular HA by hyaluronidase caused a significant decrease in the anti-Candida activity of the cells. In addition anti-Candida activity was observed at 1 micro g/ml HA (2000 kDa). The antifungal activity of extracellular HA was further studied by transiently transfecting COS-7 cells with human HSA1, HSA2, or HSA3 in order to produce high levels of extracellular HA. All of the transfectants inhibited C. albicans growth in vitro by 51-65% compared to 38% inhibition by the vector control (P<0.05). These results suggest that the anti-Candida activity of epithelial-cells is mediated by extracellular HA.
白色念珠菌在口腔黏膜中既是共生菌又是病原菌。先前的研究表明，上皮细胞相关的碳水化合物部分可抑制白色念珠菌的生长。在本研究中，通过检测透明质酸（HA）的作用，对上皮细胞抑制念珠菌生长的机制进行了研究。白色念珠菌与KB细胞或COS-7细胞共培养，在效应细胞与靶细胞（E:T）比例为80:1时，可使真菌的体外生长受到50 - 87%的抑制。用透明质酸酶去除细胞外HA会导致细胞抗念珠菌活性显著降低。此外，在1μg/ml HA（2000 kDa）时观察到了抗念珠菌活性。为了产生高水平的细胞外HA，通过用人HSA1、HSA2或HSA3瞬时转染COS-7细胞，进一步研究了细胞外HA的抗真菌活性。与载体对照的38%抑制率相比，所有转染子在体外均使白色念珠菌的生长受到51 - 65%的抑制（P<0.05）。这些结果表明，上皮细胞的抗念珠菌活性是由细胞外HA介导的。
