Zhang Fanfan, Chen Yangyang, Zhang Dan, Jia Yang, Meng Junsheng, Jiang Lirong, Yang Shengke
Key Laboratory of Subsurface Hydrology and Ecology in Arid Areas, Ministry of Education, Chang'an University, Xi'an 710054, China.
School of Water and Environment, Chang'an University, Xi'an 710054, China.
Int J Anal Chem. 2022 Aug 27;2022:6970747. doi: 10.1155/2022/6970747. eCollection 2022.
A simple, rapid, and convenient method for the determination of neomycin based on the ion association method was proposed. In Britton-Robinson buffer solution, neomycin could react with Congo red to form an ionic association, which in turn reacted with dodecyl trimethyl ammonium bromide to form a ternary ionic association. The three were combined in a 1 : 1 : 1 ratio, which significantly enhanced the resonant Rayleigh scattering intensity at 468 nm. The obtained resonant Rayleigh scattering sensor showed a linear relationship with neomycin in the range of 0.07∼1 g·mL. The limit of detection was 0.02 g·mL, and the limit of quantification was 0.037 g·mL. The experimental conditions were optimized. The method was verified based on the ICH rule. The established method could be applied to the analysis of the acceptable recovery rate of neomycin in powdered veterinary drugs.
提出了一种基于离子缔合方法测定新霉素的简单、快速且便捷的方法。在Britton-Robinson缓冲溶液中，新霉素可与刚果红反应形成离子缔合物，该离子缔合物又与十二烷基三甲基溴化铵反应形成三元离子缔合物。三者以1∶1∶1的比例结合，显著增强了468nm处的共振瑞利散射强度。所制得的共振瑞利散射传感器与新霉素在0.07～1g·mL范围内呈线性关系。检测限为0.02g·mL，定量限为0.037g·mL。对实验条件进行了优化。该方法依据ICH规则进行了验证。所建立的方法可应用于兽用粉剂中新霉素的回收率分析。
