以二硫苏糖醇-十二烷基苯磺酸钠为探针，用共振光散射技术测定蛋白质。
Determination of protein by resonance light scattering technique using dithiothreitol-sodium dodecylbenzene sulphonate as probe.
作者信息
Wu Lihang, Mu Dan, Gao Dejiang, Deng Xinyu, Tian Yuan, Zhang Hanqi, Yu Aimin
机构信息
College of Chemistry, Jilin University, Changchun 130012, PR China.
出版信息
Spectrochim Acta A Mol Biomol Spectrosc. 2009 Feb;72(1):178-81. doi: 10.1016/j.saa.2008.09.022. Epub 2008 Oct 14.
The resonance light scattering (RLS) spectra of bovine serum albumin (BSA)-dithiothreitol (DTT)-sodium dodecylbenzene sulphonate (SDBS) and its analytical application were investigated. The RLS intensity of this system can be effectively enhanced in the presence of BSA. Based on the enhanced RLS intensity, a simple assay for BSA was developed. The experimental results indicate that the enhanced RLS intensity is proportional to the concentration of BSA in the range from 1.0 x 10(-8) to 7.5 x 10(-7) mol L(-1) with the determination limit of 5.0 x 10(-9) mol L(-1). The effects of pH, concentration of SDBS and DTT on the RLS enhancement were discussed. Most metal ions have little interference on the determination of BSA. Some synthetic and real samples were analyzed, and the results obtained were in good agreement with those obtained by Bradford method.
研究了牛血清白蛋白（BSA）-二硫苏糖醇（DTT）-十二烷基苯磺酸钠（SDBS）体系的共振光散射（RLS）光谱及其分析应用。在BSA存在下，该体系的RLS强度可有效增强。基于增强的RLS强度，建立了一种简单的BSA测定方法。实验结果表明，在1.0×10⁻⁸至7.5×10⁻⁷mol L⁻¹范围内，增强的RLS强度与BSA浓度成正比，测定限为5.0×10⁻⁹mol L⁻¹。讨论了pH值、SDBS和DTT浓度对RLS增强的影响。大多数金属离子对BSA的测定干扰很小。分析了一些合成样品和实际样品，所得结果与Bradford法所得结果吻合良好。
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