配体-蛋白质相互作用数据解读中的一个陷阱。
A pitfall in the interpretation of data on ligand-protein interaction.
作者信息
Swillens S, Dumont J E
出版信息
Biochem J. 1975 Sep;149(3):779-82. doi: 10.1042/bj1490779.
Abstract
Binding reactions are usually investigated by separation of free and bound labelled ligand. Underestimation of the amount bound leads to inaccurate estimation of the dissociation constant and to wrong conclusions on the validity of the model of binding. The activation of cyclic AMP-dependent protein kinase is treated as an example.
摘要
结合反应通常通过分离游离的和结合的标记配体来进行研究。对结合量的低估会导致解离常数的估计不准确，并对结合模型的有效性得出错误结论。以环磷酸腺苷依赖性蛋白激酶的激活为例进行说明。
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