胆酸与大鼠肝脏100000g上清液制剂相互作用的平衡透析研究。
Equilibrium-dialysis studies of the interaction between cholic acid and 100000g-supernatant preparations from the rat liver.
作者信息
Strange R C, Nimmo I A, Percy-Robb I W
出版信息
Biochem J. 1976 May 15;156(2):427-33. doi: 10.1042/bj1560427.
Abstract
- The binding of cholic acid to 100000g supernatants from rat livers was investigated by equilibrium dialysis and gel-exculsion chromatography. 2. Supernatants were found to contain at least two classes of binding site for cholic acid. 3. These recptor molecules are probably proteins since incubation with proteolytic enzymes resulted in complete loss of cholic acid binding. 4. Supernatants were added to columns of Sephadex G-75, and two groups of fractions were shown to bind cholic acid. One of these contained low-affinity binding sites and the other contained both low- and high-affinity binding sites. 5. Feeding cholestyramine had no effect on cholic acid binding. 6. Increased cholic acid binding occurred after injection of phenobarbitone. There was an increase in the amount of the low-affinity component but no change in the high-affinity component. 7. The dissociation constants of the binding of cholic acid suggest that the binding proteins may be involved in bile acid transport.
摘要
- 通过平衡透析和凝胶排阻色谱法研究了胆酸与大鼠肝脏100000g上清液的结合情况。2. 发现上清液中至少含有两类胆酸结合位点。3. 这些受体分子可能是蛋白质，因为用蛋白水解酶孵育会导致胆酸结合完全丧失。4. 将上清液加入到葡聚糖凝胶G-75柱中，结果显示有两组级分能结合胆酸。其中一组含有低亲和力结合位点，另一组含有低亲和力和高亲和力结合位点。5. 喂食消胆胺对胆酸结合没有影响。6. 注射苯巴比妥后胆酸结合增加。低亲和力组分的量增加，但高亲和力组分没有变化。7. 胆酸结合的解离常数表明，结合蛋白可能参与胆汁酸的转运。
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