关于使用平衡透析法量化配体与大分子结合方式的评估。
An evaluation of ways of using equilibrium dialysis to quantify the binding of ligand to macromolecule.
作者信息
Nimmo I A, Atkins G L, Strange R C, Percy-Robb I W
出版信息
Biochem J. 1977 Jul 1;165(1):107-10. doi: 10.1042/bj1650107.
Abstract
- The effect of systematic error (loss of ligand, complex or macromolecule) on three of the experimental designs by which equilibrium dialysis may be used to quantify the interaction of ligand and macromolecule is examined theoretically, and the design that is least sensitive to systematic error is identified. 2. Thirteen methods for fitting the binding isotherm to experimental data are compared by using them to analyse simulated data containing random error, and the most reliable method is identified.
摘要
- 从理论上研究了系统误差（配体、复合物或大分子的损失）对平衡透析用于定量配体与大分子相互作用的三种实验设计的影响，并确定了对系统误差最不敏感的设计。2. 通过使用十三种将结合等温线拟合到实验数据的方法来分析包含随机误差的模拟数据，对这些方法进行了比较，并确定了最可靠的方法。
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