结核分枝杆菌起始因子1的克隆、表达、纯化、结晶及初步X射线晶体学分析
Cloning, expression, purification, crystallization and preliminary X-ray crystallographic analysis of initiation factor 1 from Mycobacterium tuberculosis.
作者信息
Hatzopoulos Georgios N, Mueller-Dieckmann Jochen
机构信息
EMBL Hamburg Outstation, c/o DESY, Notkestrasse 85, D-22603 Hamburg, Germany.
出版信息
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Mar 1;63(Pt 3):183-6. doi: 10.1107/S174430910700437X. Epub 2007 Feb 10.
Abstract
Initiation factor 1 (IF-1; Rv3462c) from Mycobacterium tuberculosis, a component of the 30S initiation complex, was cloned and heterologously expressed in Escherichia coli. The protein was purified by affinity and size-exclusion chromatography and crystallized. A complete data set has been collected to high resolution. The crystals belonged to space group P2(1)2(1)2, with two molecules per asymmetric unit which are related by translational symmetry.
摘要
来自结核分枝杆菌的起始因子1（IF-1；Rv3462c）是30S起始复合物的一个组成部分，已在大肠杆菌中克隆并异源表达。该蛋白通过亲和色谱和尺寸排阻色谱纯化并结晶。已收集到完整的高分辨率数据集。晶体属于空间群P2(1)2(1)2，每个不对称单元中有两个分子，它们通过平移对称性相关。
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