[骨形态发生蛋白-2对兔下颌骨牵张成骨的影响]

[Effect of bone morphogenetic proteins-2 on mandibular distraction osteogenesis in rabbits].

作者信息

Chen An-wei, Wei Feng-cai, Wang Ke-tao, Sun Shan-zhen, Liu Shao-hua

机构信息

Department of Oral and Maxillofacial Surgery, Qilu Hospital of Shandong University, Jinan Shandong 250012, China.

出版信息

Zhonghua Kou Qiang Yi Xue Za Zhi. 2007 Jan;42(1):15-7.

PMID:17331435

Abstract

OBJECTIVE

To investigate the Effect of recombinant adenovirus vectors containing human Bone morphogenetic proteins-2 (Ad-hBMP-2) on the for mandibular distraction osteogenesis (DO) in rabbits.

METHODS

Twenty-four New Zealand white rabbits were randomly divided into experimental group, and control group and underwent mandibular distraction osteogenesis. After 5 days latency, the distracters were activated at a speed of 0.5 mm every 12 hours for 7 days, then on the first day in the consolidation period, the distraction gaps of experimental group were injected with 0.2 ml Ad-hBMP2 10(12) pfu/L, while the animals of control group were injected with 0.2 ml Ad-EGFP 10(12) pfu/L. At the 7 th and 28 th day of consolidation period, specimens were obtained, X-ray and histomorphology were performed. The bone density and the quantity of new bone formation in the distraction gaps were observed and compared between the two groups at different consolidation period.

RESULTS

Ad-hBMP-2 treated specimens demonstrated an increased amount of new bone formation

CONCLUSIONS

Adenovirally-mediated delivery of BMP-2 can locally increase bone deposition during DO, which may potentially shorten the consolidation period.

摘要

目的

研究携带人骨形态发生蛋白-2的重组腺病毒载体（Ad-hBMP-2）对兔下颌骨牵张成骨的影响。

方法

将24只新西兰白兔随机分为实验组和对照组，进行下颌骨牵张成骨。经过5天的潜伏期后，以每12小时0.5毫米的速度激活牵张器，持续7天，然后在巩固期的第一天，向实验组的牵张间隙注射0.2毫升10(12) pfu/L的Ad-hBMP2，而对照组动物注射0.2毫升10(12) pfu/L的Ad-EGFP。在巩固期的第7天和第28天，获取标本，进行X射线和组织形态学检查。观察并比较两组在不同巩固期牵张间隙的骨密度和新骨形成量。