Pampu A Alper, Ozkaynak Ozkan, Senel Figen Cizmeci, Cankaya Mustafa, Dolanmaz Doğan, Ersöz Safak, Avunduk Mustafa Cihat
Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Karadeniz Technical University, Trabzon, Turkey.
Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2009 Dec;108(6):833-7. doi: 10.1016/j.tripleo.2009.07.028. Epub 2009 Oct 14.
The aim of this experimental study was to evaluate the effects of osteoformin on mineralization and quality of newly formed bone during distraction osteogenesis in rabbits.
Distraction rate was as 1 mm/day for 5 days. In the experimental group, 100 microg/kg osteoformin in 100 microL/kg phosphate-buffered saline (PBS) was injected into the distraction area at day 1 and day 7 after surgery. In the control group, only 100 microL/kg PBS was injected on the same days. On day 42, the animals were killed.
Bone mineral density and bone mineral content values increased significantly in the experimental group (P < .05). Newly formed bone areas, number of vessels and osteoblasts were significantly greater in experimental group (P < .05). The number of fibroblasts were also greater, but it was not significant statistically (P > .05). The number of osteoclasts was significantly lower in experimental group (P < .05).
The results of this study show that osteoformin improves healing of regenerate in distraction osteogenesis in rabbits.
本实验研究旨在评估骨形成素对兔牵张成骨过程中新形成骨的矿化及质量的影响。
牵张速率为每天1毫米，持续5天。实验组在术后第1天和第7天于牵张区域注射100微克/千克骨形成素，溶于100微升/千克磷酸盐缓冲盐水（PBS）中。对照组在相同日期仅注射100微升/千克PBS。在第42天，处死动物。
实验组的骨密度和骨矿物质含量值显著增加（P < 0.05）。实验组新形成的骨面积、血管数量和成骨细胞数量显著更多（P < 0.05）。成纤维细胞数量也更多，但在统计学上无显著差异（P > 0.05）。实验组破骨细胞数量显著更低（P < 0.05）。
本研究结果表明，骨形成素可改善兔牵张成骨中再生骨的愈合。
