Martin Benoît, Lapouble Eve, Chaix Yohan
Laboratoire de Neurobiologie, Université d'Orléans, Orléans Cedex 2, France.
ScientificWorldJournal. 2007 Jan 26;7:83-6. doi: 10.1100/tsw.2007.40.
The mutation extra-toes (Gli3Xt-J) on chromosome (Chr) 13 of the mouse is known to be involved in the development of the skeleton. The only visible manifestation is the presence of an extra digit on each hind foot. Here we report evidence from several experiments that Gli3XtJ/+ mice weigh more than littermate Gli3+/+ mice, suggesting an effect on body weight of Gli3 or of a gene tightly linked to it on Chr 13. Four independent experiments in different environments were conducted on mice with different genetic backgrounds derived from the C3XtEso Gli3Xt-J/+ Eso/+ linkage testing strain and the JE/Le strain at adult age. The analyses have shown an association between the Gli3Xt-J allele and a body weight increase of about 6.5%. This effect is genetically dominant. It would appear that if the gene of interest is not Gli3 itself, it must be very close to this locus. Indeed, the expected size for this fragment is 7.9 +/- 5.3 cM. The manifestation of this gene, observed in two animal facilities and on different genetic backgrounds, is consistent with the idea that the effect of the gene(s) is displayed in a stable manner. It accounts for a variation of 6.5% of body weight.
已知小鼠13号染色体上的额外脚趾突变（Gli3Xt-J）与骨骼发育有关。唯一可见的表现是每只后足多了一个脚趾。在此，我们报告来自多个实验的证据，表明Gli3XtJ/+小鼠比同窝的Gli3+/+小鼠体重更重，这表明Gli3或13号染色体上与其紧密连锁的一个基因对体重有影响。在成年期，对来自C3XtEso Gli3Xt-J/+ Eso/+连锁测试品系和JE/Le品系、具有不同遗传背景的小鼠在不同环境中进行了四项独立实验。分析表明，Gli3Xt-J等位基因与体重增加约6.5%之间存在关联。这种效应是遗传显性的。似乎如果感兴趣的基因不是Gli3本身，那么它一定非常接近这个基因座。实际上，这个片段的预期大小是7.9 +/- 5.3厘摩。在两个动物设施中以及在不同遗传背景下观察到的这个基因的表现，与该基因（或这些基因）的效应以稳定方式表现的观点一致。它占体重变化的6.5%。
