Institute of Cellular and Molecular Biology, Jiangsu Normal University, No. 101 Shanghai Road, Xuzhou, 221116, Jiangsu, People's Republic of China.
Mol Biol Rep. 2013 Feb;40(2):1911-5. doi: 10.1007/s11033-012-2247-y. Epub 2012 Oct 25.
Gli3 is a zinc finger transcription factor which plays a critical role in regulating animal development, metabolism and energy partitioning and thus has the potential to influence economical important traits in farm animals. In this study, we screened the complete exons of the caprine Gli3 gene using PCR-SSCP methods in 430 individuals from three goat breeds to identify sequence variants that might be associated with growth traits. Six novel mutations (GU363952:g.739C>G, 749A>T, 1636C>A, 1982delT, 1983T>C, 2856T>C) were identified. Significant associations were observed between the mutations GU363952:g.739C>G and g.749A>T with body height, chest circumference and canon circumference. Individuals with genotype G4-CC/AA and G4-CG/AT were significantly higher than individuals with genotype G4-GG/TT in body height, chest circumference and canon circumference. The results of this study suggested that the Gli3-gene-specific SNP could be a useful marker for growth traits in future marker-assisted selection programs in goat.
Gli3 是一种锌指转录因子，在调节动物发育、代谢和能量分配方面起着关键作用，因此有可能影响农场动物的重要经济性状。在这项研究中，我们使用 PCR-SSCP 方法在三个山羊品种的 430 个个体中筛选了山羊 Gli3 基因的完整外显子，以鉴定可能与生长性状相关的序列变异。鉴定出了六个新的突变（GU363952：g.739C>G、749A>T、1636C>A、1982delT、1983T>C、2856T>C）。突变 GU363952：g.739C>G 和 g.749A>T 与体高、胸围和管围之间存在显著关联。基因型 G4-CC/AA 和 G4-CG/AT 的个体显著高于基因型 G4-GG/TT 的个体在体高、胸围和管围方面。这项研究的结果表明，Gli3 基因特异性 SNP 可以成为未来山羊标记辅助选择计划中生长性状的有用标记。
