Pessková V, Kubies D, Hulejová H, Himmlová L
Rheumatological Institute, Na Slupi 4, Prague 2, Prague, Czech Republic.
J Mater Sci Mater Med. 2007 Mar;18(3):465-73. doi: 10.1007/s10856-007-2006-0.
Interactions of the foreign material of implant and the living tissue on the cell level can cause prolonged healing or, worse, loss of the implant. The cell response to the presence of some implant materials was studied under in vitro conditions. The influence of physicochemical surface parameters on the response of the cells in the immediate vicinity of implants, namely on adhesion, proliferation and synthetic activity of fibroblasts, and on the blood coagulation were compared. The direct contact of tested materials (titanium and Ti6Al4V alloy with various surface treatments, Cr Co Mo alloy, hydroxyapatite-coated titanium, zirconium oxide ceramics, polyethylene and carbon composite) on cell spreading was monitored and the presence of TNF-alpha and IL-8 was evaluated in the cultivation medium. The formation of blood clots was investigated on samples immersed in a well with freshly drawn whole rabbit blood using a scanning electron microscope. The surface free energy was estimated using the measurement of static contact angle. Both the advancing and receding contact angles were measured by the dynamic Wilhemy plate method. Two main groups with extremes in cell viability were established. In the first group the increased polar component of surface free energy, the highest cell density, the lowest inflammatory cytokine production, but no fibres in the clotting blood were found. On the contrary, the second group of materials with a very low polar component of the surface free energy showed distinctly higher expression of inflammatory mediators, low cell proliferation, but faster formation of fibres in the blood coagulum.
植入物的外来材料与活组织在细胞水平上的相互作用可能导致愈合时间延长，或者更糟的是，植入物丢失。在体外条件下研究了细胞对某些植入材料存在的反应。比较了物理化学表面参数对植入物紧邻区域细胞反应的影响，即对成纤维细胞的黏附、增殖和合成活性以及对血液凝固的影响。监测了测试材料（经过各种表面处理的钛和Ti6Al4V合金、CrCoMo合金、羟基磷灰石涂层钛、氧化锆陶瓷、聚乙烯和碳复合材料）与细胞铺展的直接接触情况，并评估了培养基中TNF-α和IL-8的存在情况。使用扫描电子显微镜研究了浸泡在装有新鲜抽取的全兔血的孔中的样品上血栓的形成情况。通过测量静态接触角来估计表面自由能。前进接触角和后退接触角均通过动态Wilhemy板法测量。确定了细胞活力处于极端状态的两个主要组。在第一组中，发现表面自由能的极性成分增加、细胞密度最高、炎性细胞因子产生最低，但凝血中没有纤维。相反，第二组材料的表面自由能极性成分非常低，显示出炎性介质的表达明显更高、细胞增殖低，但血凝块中纤维形成更快。
