Liu Xiangyong, Zhang Xiaohua, Wang Chao, Liu Liangyu, Lei Ming, Bao Xiaoming
State Key Laboratory of Microbial Technology, Shandong University, Jinan, 250100, P.R. China,
Curr Microbiol. 2007 Apr;54(4):325-30. doi: 10.1007/s00284-006-0525-4. Epub 2007 Mar 2.
The Saccharomyces cerevisiae BDF1 gene, which encodes a bromodomain-containing transcription factor, was previously isolated by transposon mutugenesis in a screen for salt-sensitive mutants. However, the salt stress response mechanism regulated by bromodomain transcription factor 1 protein (Bdf1p) remains poorly understood. In this report, genetic analysis indicated that the salt sensitivity of the BDF1 deletion mutant was suppressed by increased gene dosage of its homologous gene BDF2. Furthermore, comparative transcriptome analysis revealed that the differences in transcriptional response between the wild type and the bdf1Delta mutant in the presence of salt stress (0.6 mol/L NaCl, 45 min) were mainly related to cell-wall biosynthesis, the mitochondria, and several unknown genes. Our results provided further information about the regulatory mechanism involved in the salt stress response and adds new insight for understanding the biological functional of bromdomain-containing proteins in cellular processes.
酿酒酵母BDF1基因编码一种含溴结构域的转录因子，该基因先前是在盐敏感突变体筛选中通过转座子诱变分离得到的。然而，由溴结构域转录因子1蛋白（Bdf1p）调控的盐胁迫应答机制仍知之甚少。在本报告中，遗传分析表明，BDF1缺失突变体的盐敏感性可通过增加其同源基因BDF2的基因剂量来抑制。此外，比较转录组分析显示，在盐胁迫（0.6 mol/L NaCl，45分钟）下，野生型和bdf1Δ突变体之间转录应答的差异主要与细胞壁生物合成、线粒体以及几个未知基因有关。我们的结果提供了关于盐胁迫应答调控机制的更多信息，并为理解含溴结构域蛋白在细胞过程中的生物学功能增添了新的见解。
