Norman R J, Gilmore T A, McLoughlin J W
University of Adelaide, Department of Obstetrics and Gynaecology, Queen Elizabeth Hospital, Woodville, South Australia.
Clin Chem. 1992 Jan;38(1):144-7.
We evaluated a new simplified quantitative method (Tandem Icon QSR; Hybritech) for choriogonadotropin (hCG), which could theoretically be performed in a ward or with outpatients. The method was compared with immunoradiometric (Bioclone Australia), chemiluminescent (Amerlite; Amersham), and immunoenzymometric assays (Stratus; Dade). We analyzed by each of the methods 104 serum samples from pregnant and nonpregnant patients. For quantitative hCG values indicative of pregnancy (greater than 25 int. units/L), excellent correlation was observed between the Tandem Icon method and the other standard laboratory assays (r = 0.995, 0.990, and 0.992, respectively). Occasional problems arose because of the instability of Tandem Icon QSR reagents at room temperature but this was resolved by storing the reagents at 4 degrees C. We conclude that this simplified quantitative method for hCG is reliable and suitable for use outside of the routine immunoassay laboratory.
我们评估了一种新的简化定量方法（串联免疫分析系统QSR；Hybritech公司）用于检测绒毛膜促性腺激素（hCG），理论上该方法可在病房或门诊对患者进行检测。将该方法与免疫放射分析法（澳大利亚Bioclone公司）、化学发光分析法（Amerlite；Amersham公司）以及免疫酶分析法（Stratus；Dade公司）进行了比较。我们用这几种方法对104例来自孕妇和非孕妇的血清样本进行了分析。对于指示妊娠的定量hCG值（大于25国际单位/升），串联免疫分析系统方法与其他标准实验室检测方法之间观察到极好的相关性（相关系数分别为0.995、0.990和0.992）。由于串联免疫分析系统QSR试剂在室温下不稳定，偶尔会出现问题，但通过将试剂储存在4℃得以解决。我们得出结论，这种简化的hCG定量方法可靠，适用于常规免疫分析实验室之外的检测。
