Alexander R L, Mueller D S, Lipe A L, Minton J S, Szmurlo R E
Clin Chem. 1986 Jul;32(7):1404-7.
We evaluated the Hybritech Photon Analyzer for its applicability to enzyme immunoassays. This instrument measures absorbances only at 405, 450, and 490 nm. Photometric linearity was good at all three wavelengths. However, the true absorbance range at these wavelengths was 0-1.6 A instead of 0-2.0 A as specified by the manufacturer. We also evaluated the Hybritech "Tandem-E" method for quantifying choriogonadotropin (hCG) in serum. A modification of the recommended procedure improved the precision of the assay. The detection limit of the modified procedure, 1 int. unit/L, permits reliable assays of hCG at concentrations near the upper limit of normal (3 int. units/L). Results by both procedures varied linearly with hCG concentrations up to 250 int. units/L. The Photon Analyzer eliminates the need for manual calculations. Quantitative results agree within +/- 1% with those measured in the "Stasar III" spectrophotometer.
我们评估了Hybritech光子分析仪在酶免疫测定中的适用性。该仪器仅在405、450和490纳米处测量吸光度。在所有这三个波长处，光度线性良好。然而，这些波长处的实际吸光度范围是0至1.6吸光度单位，而非制造商规定的0至2.0吸光度单位。我们还评估了Hybritech“串联 - E”法用于定量血清中绒毛膜促性腺激素（hCG）的情况。对推荐程序的修改提高了测定的精密度。修改后程序的检测限为1国际单位/升，能够对接近正常上限（3国际单位/升）浓度的hCG进行可靠测定。两种程序的结果在hCG浓度高达250国际单位/升时均与hCG浓度呈线性变化。光子分析仪无需手动计算。定量结果与在“Stasar III”分光光度计中测量的结果在±1%范围内一致。
