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我将存活:细菌芽孢中的DNA保护

I will survive: DNA protection in bacterial spores.

作者信息

Setlow Peter

机构信息

Department of Molecular, Microbial and Structural Biology, University of Connecticut Health Center, Farmington, CT 06030-3305, USA.

出版信息

Trends Microbiol. 2007 Apr;15(4):172-80. doi: 10.1016/j.tim.2007.02.004. Epub 2007 Mar 1.

Abstract

Dormant spores of Bacillus, Clostridium and related species can survive for years, largely because spore DNA is well protected against damage by many different agents. This DNA protection is partly a result of the high level of Ca(2+)-dipicolinic acid in spores and DNA repair during spore outgrowth, but is primarily caused by the saturation of spore DNA with a group of small, acid-soluble spore proteins (SASP), which are synthesized in the developing spore and then degraded after completion of spore germination. The structure of both DNA and SASP alters upon their association and this causes major changes in the chemical and photochemical reactivity of DNA.

摘要

芽孢杆菌属、梭菌属及相关菌种的休眠孢子可存活数年,这主要是因为孢子DNA受到多种不同因素的保护,不易受损。这种DNA保护部分归因于孢子中高水平的Ca(2+) - 吡啶二羧酸以及孢子萌发过程中的DNA修复,但主要是由一组小的、酸溶性孢子蛋白(SASP)使孢子DNA饱和所致,这些蛋白在发育中的孢子中合成,在孢子萌发完成后降解。DNA和SASP结合后,二者的结构都会改变,这会导致DNA的化学和光化学反应性发生重大变化。

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