通过电子断层扫描分析裂殖酵母中分裂间期微管的组织。

Organization of interphase microtubules in fission yeast analyzed by electron tomography.

作者信息

Höög Johanna L, Schwartz Cindi, Noon Angela T, O'Toole Eileen T, Mastronarde David N, McIntosh J Richard, Antony Claude

机构信息

European Molecular Biology Laboratory, Cell Biology and Biophysics Program, D-69117 Heidelberg, Germany.

出版信息

Dev Cell. 2007 Mar;12(3):349-61. doi: 10.1016/j.devcel.2007.01.020.

DOI:10.1016/j.devcel.2007.01.020

PMID:17336902

Abstract

Polarized cells, such as neuronal, epithelial, and fungal cells, all display a specialized organization of their microtubules (MTs). The interphase MT cytoskeleton of the rod-shaped fission yeast, Schizosaccharomyces pombe, has been extensively described by fluorescence microscopy. Here, we describe a large-scale, electron tomography investigation of S. pombe, including a 3D reconstruction of a complete eukaryotic cell volume at sufficient resolution to show both how many MTs there are in a bundle and their detailed architecture. Most cytoplasmic MTs are open at one end and capped at the other, providing evidence about their polarity. Electron-dense bridges between the MTs themselves and between MTs and the nuclear envelope were frequently observed. Finally, we have investigated structure/function relationships between MTs and both mitochondria and vesicles. Our analysis shows that electron tomography of well-preserved cells is ideally suited for describing fine ultrastructural details that were not visible with previous techniques.

摘要

极化细胞，如神经元细胞、上皮细胞和真菌细胞，均呈现出其微管（MTs）的特殊组织形式。杆状裂殖酵母（Schizosaccharomyces pombe）的间期MT细胞骨架已通过荧光显微镜进行了广泛描述。在此，我们描述了一项对粟酒裂殖酵母的大规模电子断层扫描研究，包括对一个完整真核细胞体积进行三维重建，其分辨率足以显示一束中有多少MTs及其详细结构。大多数细胞质MTs一端开放，另一端封闭，这为其极性提供了证据。经常观察到MTs自身之间以及MTs与核膜之间存在电子致密桥。最后，我们研究了MTs与线粒体和囊泡之间的结构/功能关系。我们的分析表明，保存良好的细胞的电子断层扫描非常适合描述以前技术无法看到的精细超微结构细节。