Centre for BioSystems Science and Engineering, Indian Institute of Science, Bengaluru 560012, India.
Cell Biology and Biophysics Unit, European Molecular Biology Laboratory, 69117 Heidelberg, Germany.
J Cell Sci. 2023 Jan 1;136(1). doi: 10.1242/jcs.260705. Epub 2023 Jan 12.
Association with microtubules inhibits the fission of mitochondria in Schizosaccharomyces pombe. Here, we show that this attachment of mitochondria to microtubules is an important cell-intrinsic factor in determining cell division symmetry. By comparing mutant cells that exhibited enhanced attachment and no attachment of mitochondria to microtubules (Dnm1Δ and Mmb1Δ, respectively), we show that microtubules in these mutants displayed aberrant dynamics compared to wild-type cells, which resulted in errors in nuclear positioning. This translated to cell division asymmetry in a significant proportion of both Dnm1Δ and Mmb1Δ cells. Asymmetric division in Dnm1Δ and Mmb1Δ cells resulted in unequal distribution of mitochondria, with the daughter cell that received more mitochondria growing faster than the other daughter cell. Taken together, we show the existence of homeostatic feedback controls between mitochondria and microtubules in fission yeast, which directly influence mitochondrial partitioning and, thereby, cell growth. This article has an associated First Person interview with the first author of the paper.
线粒体与微管的结合抑制了裂殖酵母中线粒体的裂变。在这里，我们表明，线粒体与微管的这种附着是决定细胞分裂对称性的一个重要的细胞内在因素。通过比较表现出线粒体与微管附着增强和无附着的突变细胞（分别为 Dnm1Δ 和 Mmb1Δ），我们表明，与野生型细胞相比，这些突变体中的微管显示出异常的动力学，这导致核定位错误。这导致 Dnm1Δ 和 Mmb1Δ 细胞中相当一部分出现细胞分裂不对称。在 Dnm1Δ 和 Mmb1Δ 细胞中不对称分裂导致线粒体分布不均，接收更多线粒体的子细胞比另一个子细胞生长得更快。总之，我们展示了裂殖酵母中线粒体和微管之间存在一种动态的反馈控制，这种控制直接影响线粒体的分配，从而影响细胞的生长。本文附有该论文第一作者的第一人称采访。
