Matsumoto Kenji, Watanabe Yutaka, Yokoyama Shin-ichiro
Gifu Prefectural Research Institute for Bioengineering, Department of Applied Microbiology, Minokamo, Gifu, Japan.
Biosci Biotechnol Biochem. 2007 Mar;71(3):720-7. doi: 10.1271/bbb.60563. Epub 2007 Mar 7.
We examined the effect of okara on the prevention of obesity in mice. A modified AIN-76 diet with a high fat content (14.1% of crude fat) was used as a basal diet. Male ICR mice were fed ad libitum with the basal diet or a dried okara-supplemented basal diet (10, 20, or 40%) for 10 weeks. The okara intake dose-dependently suppressed the development of body weight and epididymal white adipose tissue (EWAT), and prevented an increase of plasma lipids, including total cholesterol, LDL cholesterol, and non-esterified fatty acid. The okara intake also prevented steatosis in the liver. Real-time RT-PCR revealed that the okara intake induced down-regulation of the fatty acid synthetase gene and up-regulation of the cholesterol 7 alpha-hydroxylase (CYP7A1) gene in the liver. We also found that the okara intake caused a marked reduction in the expression of leptin and TNF-alpha genes in EWAT. Our results suggest that okara is beneficial in preventing obesity.
我们研究了豆渣对预防小鼠肥胖的作用。以高脂肪含量（粗脂肪含量为14.1%）的改良AIN - 76日粮作为基础日粮。雄性ICR小鼠自由采食基础日粮或添加不同比例（10%、20%或40%）干燥豆渣的基础日粮，持续10周。豆渣摄入量呈剂量依赖性地抑制体重增加和附睾白色脂肪组织（EWAT）的生长，并防止包括总胆固醇、低密度脂蛋白胆固醇和非酯化脂肪酸在内的血脂升高。摄入豆渣还可预防肝脏脂肪变性。实时逆转录聚合酶链反应（Real-time RT-PCR）显示，摄入豆渣可导致肝脏中脂肪酸合成酶基因下调以及胆固醇7α - 羟化酶（CYP7A1）基因上调。我们还发现，摄入豆渣会使EWAT中瘦素和肿瘤坏死因子-α（TNF-α）基因的表达显著降低。我们的研究结果表明，豆渣对预防肥胖有益。
