Vandenberg Olivier, Van Laethem Yves, Souayah Hichem, Kutane Waltaji Terfa, van Gool Tom, Dediste Anne
Department of Microbiology, Saint-Pierre University Hospital, Brussels, Belgium.
Acta Gastroenterol Belg. 2006 Oct-Dec;69(4):361-6.
To perform optimal laboratory diagnosis of intestinal parasites is demanding. Because intestinal parasites are intermittently shedded, examination of multiple stools is imperative. For reliable detection of vegetative stages of protozoa, fresh stools should be examined direct after production, or stools should be preserved in a fixative. These aspects in routine practice are often neglected with as a result lower sensitivity of the diagnostic procedure. With application of the Triple-Faeces-Test (TFT) protocol, where both multiple sampling and a SAF-fixative are included, these practical problems could be overcome. The aim of this study was to compare the recovery of intestinal parasites in faecal specimens using TFT protocol versus the conventional diagnostic method (ether-sedimentation of one fresh stool sample).
During a three years period, results obtained in routine practice with the TFT protocol were compared with results from examination of sediment obtained with the ethyl-acetate-sedimentation technique of one unpreserved faeces specimen.
From 2776 patients, 28.1% were positive for one or more intestinal parasites after examination of the TFT test, compared to 10.3% positivity with the conventional method (P < 0.05). Pathogenic species and non pathogenic species were observed respectively 191 and 449 times with TFT and 105 and 152 times with conventional method (P < 0.05).
The application of the Triple-Faeces-Test in routine clinical practice significantly increased recovery of intestinal parasitic infections.
进行肠道寄生虫的最佳实验室诊断颇具挑战性。由于肠道寄生虫是间歇性排出的，因此必须对多份粪便进行检查。为了可靠地检测原生动物的滋养体阶段，应在粪便产生后立即直接检查新鲜粪便，或者将粪便保存在固定剂中。在常规实践中，这些方面常常被忽视，导致诊断程序的敏感性降低。通过应用三联粪便检测（TFT）方案，其中包括多次采样和使用SAF固定剂，可以克服这些实际问题。本研究的目的是比较使用TFT方案与传统诊断方法（对一份新鲜粪便样本进行乙醚沉淀）在粪便标本中肠道寄生虫的检出率。
在三年期间，将TFT方案在常规实践中获得的结果与对一份未保存粪便标本采用乙酸乙酯沉淀技术检查沉淀物所得到的结果进行比较。
在2776例患者中，TFT检测后28.1%的患者一种或多种肠道寄生虫呈阳性，而传统方法的阳性率为10.3%（P<0.05）。TFT分别观察到致病种类和非致病种类191次和449次，传统方法分别观察到105次和152次（P<0.05）。
三联粪便检测在常规临床实践中的应用显著提高了肠道寄生虫感染的检出率。
