Anejo-Okopi Joseph Aje, Okojokwu Julius Ocheme, Ebonyi Augustine Odo, Ejeliogu Emeka Uba, Isa Samson Ejiji, Audu Onyemocho, Akpakpan Edoama Edet, Nwachukwu Esther Ebere, Ifokwe Christabel Kelechi, Ali Murna, Lar Patricia, Oguche Stephen
Department of Microbiology, University of Jos, Jos, Nigeria; AIDS Prevention Initiative in Nigeria, Jos University Teaching Hospital, Jos, Nigeria.
Department of Microbiology, University of Jos, Jos, Nigeria.
Pan Afr Med J. 2016 Dec 21;25:253. doi: 10.11604/pamj.2016.25.253.10018. eCollection 2016.
is an important cause of diarrhea in children and immune-compromised individuals. Recent advances in molecular diagnostics have led to the discovery of subtype families that are thought to be more commonly associated with diarrhea. We aimed to isolate and characterize spp among children with diarrhea in Jos, Nigeria.
Stool samples were collected from165 children aged 0-5 years with diarrhea. Cryptosporidium oocysts were examined by wet mount preparation, using formalin ether and a modified acid fast staining method. DNA was extracted from positive samples using QIAamp DNA stool mini kit and PCR-RFLP assay was carried out after quantification. Genotyping and phylogenetic analysis were done to determine the subtype families and their relatedness.
From the 165 children studied, 8 (4.8%) were infected with Cryptosporidium. PCR-RFLP assay and genotype characterization found the following 6 (75%) and 2 (25.0%), with family subtypes respectively.The most common species was and the frequent subtype was 5 (62.5%).
is not an uncommon cause of diarrhea in children, with being the dominant species. Also Id is the commonest sub-family subtype. Put together, zoonotic species may be an important cause of diarrhea in children aged 0-5 years in Jos, Nigeria.
是儿童和免疫功能低下个体腹泻的重要原因。分子诊断技术的最新进展导致发现了一些亚型家族，这些家族被认为与腹泻更为常见相关。我们旨在分离并鉴定尼日利亚乔斯市腹泻儿童中的 种。
从 165 名 0 - 5 岁腹泻儿童中采集粪便样本。使用福尔马林乙醚和改良抗酸染色法通过湿片制备检查隐孢子虫卵囊。使用 QIAamp DNA 粪便微型试剂盒从阳性样本中提取 DNA，并在定量后进行 PCR - RFLP 分析。进行基因分型和系统发育分析以确定亚型家族及其亲缘关系。
在研究的 165 名儿童中，8 名（4.8%）感染了隐孢子虫。PCR - RFLP 分析和基因型鉴定发现以下情况， 分别为 6 名（75%）和 2 名（25.0%），家族亚型分别为 。最常见的种类是 ，最常见的亚型是 5 名（62.5%）。
是儿童腹泻的常见病因， 是优势种类。此外，Id 是最常见的亚家族亚型。综合来看，人畜共患种类可能是尼日利亚乔斯市 0 - 5 岁儿童腹泻的重要原因。
