Colonques J, Ceron J, Tejedor F J
Instituto de Neurociencias, CSIC--Universidad Miguel Hernandez, Campus de San Juan, 03550 San Juan (Alicante), Spain.
Mech Dev. 2007 May;124(5):327-40. doi: 10.1016/j.mod.2007.01.004. Epub 2007 Jan 21.
Due to its intermediate complexity and its sophisticated genetic tools, the larval brain of Drosophila is a useful experimental system to study the mechanisms that control the generation of cell diversity in the CNS. In order to gain insight into the neuronal and glial lineage specificity of neural progenitor cells during postembryonic brain development, we have carried an extensive mosaic analysis throughout larval brain development. In contrast to embryonic CNS development, we have found that most postembryonic neurons and glial cells of the optic lobe and central brain originate from segregated progenitors. Our analysis also provides relevant information about the origin and proliferation patterns of several postembryonic lineages such as the superficial glia and the medial-anterior Medulla neuropile glia. Additionally, we have studied the spatio-temporal relationship between gcm expression and gliogenesis. We found that gcm expression is restricted to the post-mitotic cells of a few neuronal and glial lineages and it is mostly absent from postembryonic progenitors. Thus, in contrast to its major gliogenic role in the embryo, the function of gcm during postembryonic brain development seems to have evolved to the specification and differentiation of certain neuronal and glial lineages.
由于果蝇幼虫大脑具有中等复杂性及其完善的遗传工具，它是研究中枢神经系统中控制细胞多样性产生机制的有用实验系统。为了深入了解胚胎后脑发育过程中神经祖细胞的神经元和神经胶质谱系特异性，我们在整个幼虫大脑发育过程中进行了广泛的镶嵌分析。与胚胎中枢神经系统发育不同，我们发现视叶和中枢脑的大多数胚胎后神经元和神经胶质细胞起源于分离的祖细胞。我们的分析还提供了有关几种胚胎后谱系（如表面神经胶质细胞和内侧 - 前髓质神经毡神经胶质细胞）的起源和增殖模式的相关信息。此外，我们研究了gcm表达与神经胶质生成之间的时空关系。我们发现gcm表达仅限于少数神经元和神经胶质谱系的有丝分裂后细胞，并且在胚胎后祖细胞中大多不存在。因此，与其在胚胎中的主要神经胶质生成作用相反，gcm在胚胎后脑发育过程中的功能似乎已经演变为某些神经元和神经胶质谱系的特化和分化。
