Zhou Jie-Bin, Yang Xue-Kang, Ye Qi-Fa, Ming Ying-Zi, Xia Zong-Jiang
Institute of Organ Transplantation, Third Xiangya Hospital, Central South University, Department of General Surgery, Changsha Central Hospital, Changsha 410013, China.
Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2007 Feb;32(1):54-8.
To explore the effect of extract of ginkgo biloba leaves on the precondition of liver graft in rat liver transplantation.
Male Sprague-Dawley rats were used as donors and recipients of orthotopic liver transplantation (OLT), and were randomly divided into extract of ginkgo biloba leaves group (Egb), NS control group (NS), and sham operation group (SO) according to whether the extract of ginkgo biloba leaves was injected by the venous (40 mg/kg) 1 h before the liver grafts harvesting. The rats were killed at 2 h, 6 h, and 24 h after the ischemia/reperfusion. The serum concentrations of ALT and AST were determined and the liver tissue were sampled to observe the expression of TNF-alpha and IL-1.
After the ischemia/reperfusion the serum concentration of ALT and AST and expressions of TNF-alpha and IL-1 in the hepatic tissue in the NS group significantly increased (p<0.01), and the hepatocytic morphologic change was obvious compared with the SO group. The treatment of ginkgo biloba extract significantly decreased the serum concentration of ALT and AST and the expressions of TNF-alpha and IL-1 in the hepatic tissue in EGb group compared with the NS group (p<0.01), and relieved the hepatocyte swelling and necrosis.
Ginkgo bilobA extract may decrease the release of TNF-alpha and IL-1 by inhibiting activation of kuffer cells and regulate the cell factors to protect the live.
探讨银杏叶提取物对大鼠肝移植中肝移植物预处理的作用。
以雄性Sprague-Dawley大鼠作为原位肝移植（OLT）的供体和受体，根据在肝移植物获取前1小时是否经静脉注射银杏叶提取物（40mg/kg），将其随机分为银杏叶提取物组（Egb）、生理盐水对照组（NS）和假手术组（SO）。在缺血/再灌注后2小时、6小时和24小时处死大鼠，测定血清谷丙转氨酶（ALT）和谷草转氨酶（AST）浓度，并取肝组织样本观察肿瘤坏死因子-α（TNF-α）和白细胞介素-1（IL-1）的表达。
缺血/再灌注后，NS组血清ALT和AST浓度以及肝组织中TNF-α和IL-1的表达显著升高（p<0.01），与SO组相比，肝细胞形态学改变明显。与NS组相比，银杏叶提取物处理显著降低了Egb组血清ALT和AST浓度以及肝组织中TNF-α和IL-1的表达（p<0.01），并减轻了肝细胞肿胀和坏死。
银杏叶提取物可能通过抑制枯否细胞活化来减少TNF-α和IL-1的释放，并调节细胞因子以保护肝脏。
