单独给予银杏叶提取物（EGb761）以及联合给予 FK506 可促进部分肝切除大鼠模型的肝再生。

Administration of Extract (EGb761) Alone and in Combination with FK506 Promotes Liver Regeneration in a Rat Model of Partial Hepatectomy.

机构信息

Department of Clinical Biochemistry, University of Health Sciences, Training and Research Hospital, Kayseri, Turkey.

Department of Clinical Biochemistry, Erciyes University School of Medicine, Kayseri, Turkey.

出版信息

Balkan Med J. 2018 Mar 15;35(2):174-180. doi: 10.4274/balkanmedj.2016.1830.

DOI:10.4274/balkanmedj.2016.1830

PMID:29553465

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5863256/

Abstract

BACKGROUND

Free radical damage is known to occur during liver regeneration. The extract EGb761 has antioxidant properties due to its ability to scavenge free radicals. FK506 has been widely used as an immunosuppressant that stimulates hepatocyte proliferation following partial hepatectomy.

AIMS

To explore whether EGb761 enhances liver regeneration after hepatectomy in rats, we investigated the effects of EGb761 alone and in combination with FK506 on the liver regenerative process.

STUDY DESIGN

Animal experimentation.

METHODS

A total of 75 Wistar albino rats weighing 340.08±11.66 g were randomly divided into five experimental groups: sham, control, FK506, EGb761, and FK506 + EGb761. According to the study groups, rats were administered FK506 at a dose of 0.1 mg/kg/day and EGb761 at 25 mg/kg/day three times via the intraperitoneal route. Then, two-thirds hepatectomy was performed according to the Higgins and Anderson technique in all the rats. At postoperative 48 h, 53 surviving rats were sacrificed. Serum and plasma samples were collected for analyzing thymidine kinase and oxidative stress marker levels. The regenerated liver was entirely resected, weighed, and sectioned. The mitotic index was assessed using hematoxylin-eosin staining. The extent of liver regeneration was calculated using the Child's formula. The data were statistically analyzed using ANOVA, with a significance level of 5% (p<0.05).

RESULTS

Rats who received EGb761 showed significantly higher levels of liver regeneration than those who received FK506 or FK506 + EGb761 (p<0.01). Thymidine kinase level and mitotic index were significantly higher in the EGb761 (p<0.005) and FK506 (p<0.05) groups than in the control and sham groups. In addition, the liver regeneration percentage was significantly higher in the EGb761 group than in the FK506 group (p<0.01). Myeloperoxidase and malondialdehyde levels were significantly correlated between the EGb761 and FK506 groups, even at lower levels in the EGb761 group (p<0.001).

CONCLUSION

EGb761, which is an antioxidant, reduces liver damage and stimulates liver regeneration following partial hepatectomy in rats through its anti-inflammatory and antioxidative effects.

摘要

背景

已知肝再生过程中会发生自由基损伤。银杏叶提取物 EGb761 因其清除自由基的能力而具有抗氧化特性。FK506 已被广泛用作免疫抑制剂，可刺激肝部分切除术后的肝细胞增殖。

目的

探讨 EGb761 是否增强大鼠肝切除术后的肝再生，我们研究了 EGb761 单独和与 FK506 联合使用对肝再生过程的影响。

研究设计

动物实验。

方法

将 75 只体重为 340.08±11.66g 的 Wistar 白化大鼠随机分为五组：假手术组、对照组、FK506 组、EGb761 组和 FK506+EGb761 组。根据研究组，大鼠通过腹腔途径每天给予 FK506 0.1mg/kg 和 EGb761 25mg/kg 三次。然后，根据 Higgins 和 Anderson 技术对所有大鼠进行三分之二肝切除术。术后 48 小时，53 只存活大鼠被处死。采集血清和血浆样本以分析胸苷激酶和氧化应激标志物水平。切除再生肝，称重，切片。用苏木精-伊红染色评估有丝分裂指数。Child 公式计算肝再生程度。使用 ANOVA 对数据进行统计分析，显著性水平为 5%（p<0.05）。

结果

接受 EGb761 的大鼠的肝再生水平明显高于接受 FK506 或 FK506+EGb761 的大鼠（p<0.01）。EGb761（p<0.005）和 FK506（p<0.05）组的胸苷激酶水平和有丝分裂指数明显高于对照组和假手术组。此外，EGb761 组的肝再生百分比明显高于 FK506 组（p<0.01）。EGb761 组和 FK506 组之间的髓过氧化物酶和丙二醛水平呈显著相关，即使在 EGb761 组水平较低（p<0.001）。