Zwierz Piotr, Szajda Sławomir D, Snarska Jadwiga, Supronowicz Zbigniew B, Zawadzki Paweł, Zwierz Krzysztof, Kamińsk Fabian
Department of Pharmaceutical Biochemistry, Medical University of Białystok, Poland.
Pol Merkur Lekarski. 2006 Nov;21(125):439-42.
Thyroid cancer consists 1% of all malignant neoplasms. It is not known interrelationship between concentration of TSH in blood serum and condition of thyroid cancer. Thyroid cancer is difficult for diagnosis and differentiation. Therefore it is necessary to search for biochemical markers helpful in diagnostics of thyroid cancer. Significant increase in activity of N-acetyl-beta-D-hexosaminidase and its isoenzymes A and B in serum of patients with neoplasms of kidney and pancreas suggest approporiateness of evaluation of HEX and its isoenzymes in diagnostics of thyroid cancer.
of the study--evaluation of TSH concentration and activity of HEX and its isoenzymes A and B, in serum of patients with thyroid cancer.
Blood was taken from 7 patients with thyroid cancer (6 men and 1 woman). Control consisted of 7 healthy men. In blood serum concentration of TSH was determined with immunoenzymatic method on analyzer Axsym of Abbott and expressed in microU/mL. The activity of HEX and its isoenzymes A and B was determined by method of Chatterjee et al., as modified by Zwierz et.al. Determination of HEX was performed on microplate reader ELX800 BIO-TEK. Activity of HEX, HEX A and B was expressed in pKat/mL, and specific activity in pKat/mg protein). Protein was determined by biuret method and results were expressed in mg/mL.
Concentration of HEX A activity in serum of thyroid cancer patients is significantly higherin comparison to healthymen (p = 0.0191). Also specific activity of HEX A in serum of thyroid cancer patients is significantly higher in comparison to healthy men (p = 0.0393).
甲状腺癌占所有恶性肿瘤的1%。血清促甲状腺激素（TSH）浓度与甲状腺癌病情之间的相互关系尚不清楚。甲状腺癌的诊断和鉴别诊断较为困难。因此，有必要寻找有助于甲状腺癌诊断的生化标志物。肾和胰腺肿瘤患者血清中N - 乙酰 - β - D - 己糖胺酶及其同工酶A和B的活性显著升高，提示评估己糖胺酶（HEX）及其同工酶在甲状腺癌诊断中的适用性。
评估甲状腺癌患者血清中TSH浓度以及HEX及其同工酶A和B的活性。
采集7例甲状腺癌患者（6例男性，1例女性）的血液。对照组为7名健康男性。采用免疫酶法在雅培Axsym分析仪上测定血清中TSH浓度，结果以微单位/毫升表示。按照Chatterjee等人的方法并经Zwierz等人修改后，测定HEX及其同工酶A和B的活性。在BIO - TEK ELX800酶标仪上进行HEX的测定。HEX、HEX A和B的活性以皮卡特/毫升表示，比活性以皮卡特/毫克蛋白表示。采用双缩脲法测定蛋白质，结果以毫克/毫升表示。
甲状腺癌患者血清中HEX A的活性浓度与健康男性相比显著更高（p = 0.0191）。甲状腺癌患者血清中HEX A的比活性与健康男性相比也显著更高（p = 0.0393）。
