Román Irene, Vilalta Marta, Rodriguez Julio, Matthies Annette M, Srouji Samer, Livne Erella, Hubbell Jeffrey A, Rubio Nuria, Blanco Jerónimo
Cardiovascular Research Center, Hospital de Sant Pau, Barcelona, Spain.
Biomaterials. 2007 Jun;28(17):2718-28. doi: 10.1016/j.biomaterials.2007.02.011. Epub 2007 Feb 20.
Recent developments in stem cell research have promoted a flourishing of new biomaterials and scaffolds for tissue repair. However, there is a scarcity of procedures to monitor the performance of scaffold-stem cell combinations implanted in live animals, avoiding the inherent artefacts associated with in vitro assay conditions. We report the implementation of a procedure based on the use of the luciferase gene as a cell proliferation tracer to monitor, by in vivo non-invasive imaging, the performance of stem cell-biomaterial combinations used for tissue regeneration. In a model system using immunodepressed mice we show preference of a mouse embryonic mesenchymal cell line (C3H/10T1/2) for specific implantation sites and biomaterials during a prolonged in vivo growth period (3 months). Moreover, we analyzed the safety of implanted cells using a sensitive luminometric procedure and showed that the implanted cells did not spread to other organs. Our results demonstrate the utility of this simple and resource-saving procedure in the development and screening of biomaterials for tissue engineering.
干细胞研究的最新进展推动了用于组织修复的新型生物材料和支架的蓬勃发展。然而，目前缺乏监测植入活体动物体内的支架 - 干细胞组合性能的程序，以避免与体外检测条件相关的固有假象。我们报告了一种基于使用荧光素酶基因作为细胞增殖示踪剂的程序的实施情况，通过体内无创成像来监测用于组织再生的干细胞 - 生物材料组合的性能。在一个使用免疫抑制小鼠的模型系统中，我们展示了小鼠胚胎间充质细胞系（C3H/10T1/2）在延长的体内生长周期（3个月）内对特定植入部位和生物材料的偏好。此外，我们使用灵敏的发光测定程序分析了植入细胞的安全性，并表明植入细胞未扩散到其他器官。我们的结果证明了这种简单且节省资源的程序在组织工程生物材料的开发和筛选中的实用性。
