REMA：一种用于分析多个DNA序列中限制性酶切位点的基于计算机的图谱绘制工具。
REMA: A computer-based mapping tool for analysis of restriction sites in multiple DNA sequences.
作者信息
Szubert Jan, Reiff Caroline, Thorburn Andrew, Singh Brajesh K
机构信息
Faculty of Life Sciences, The University of Manchester, Oxford Road, Manchester, M13 9PT UK.
出版信息
J Microbiol Methods. 2007 May;69(2):411-3. doi: 10.1016/j.mimet.2007.01.008. Epub 2007 Jan 24.
UNLABELLED
REMA is an interactive web-based program which predicts endonuclease cut sites in DNA sequences. It analyses multiple sequences simultaneously and predicts the number and size of fragments as well as provides restriction maps. The users can select single or paired combinations of all commercially available enzymes. Additionally, REMA permits prediction of multiple sequence terminal fragment sizes and suggests suitable restriction enzymes for maximally discriminatory results. REMA is an easy to use, web based program which will have a wide application in molecular biology research.
AVAILABILITY
REMA is written in Perl and is freely available for non-commercial use. Detailed information on installation can be obtained from Jan Szubert (jan.szubert@gmail.com) and the web based application is accessible on the internet at the URL http://www.macaulay.ac.uk/rema
CONTACT
未标注
REMA是一个基于网络的交互式程序，可预测DNA序列中的核酸内切酶切割位点。它能同时分析多个序列，预测片段的数量和大小，并提供限制酶切图谱。用户可以选择所有市售酶的单酶或双酶组合。此外，REMA还能预测多个序列末端片段的大小，并为获得最大区分度结果推荐合适的限制酶。REMA是一个易于使用的基于网络的程序，将在分子生物学研究中有广泛应用。
可用性
REMA用Perl编写，可免费用于非商业用途。有关安装的详细信息可从扬·苏伯特（jan.szubert@gmail.com）处获取，基于网络的应用程序可通过网址http://www.macaulay.ac.uk/rema在互联网上访问。
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