Cotler Scott J, Guzman Grace, Layden-Almer Jennifer, Mazzone Theodore, Layden Thomas J, Zhou Xiaohong Joe
Section of Hepatology, Department of Pathology, University of Illinois at Chicago, Chicago, Illinois 60612, USA.
J Magn Reson Imaging. 2007 Apr;25(4):743-8. doi: 10.1002/jmri.20865.
To validate an MRI technique for measuring liver fat content by calibrating MRI readings with liver phantoms and comparing MRI measurements in human subjects with estimates of liver fat content on liver biopsy specimens.
The MRI protocol consisted of fat and water imaging by selective saturation using a 3.0-T scanner. A water phantom and liver phantoms were scanned before each of 10 human subjects who underwent a liver biopsy to assess for nonalcoholic fatty liver disease (NAFLD). Liver fat content in human subjects was derived from a calibration curve generated by scanning the phantoms. Liver fat was also estimated by optical image analysis and pathologists' assessment of histologic sections.
MRI measurements of the liver phantoms were highly reproducible. Measurements of liver fat content in human subjects made by MRI in two areas of the liver were strongly correlated (r=0.98, P<0.001). MRI measurements were highly associated with estimates of liver fat content made by optical image analysis (r=0.96, P<0.001) and with estimates made by the pathologists (r=0.93, P<0.001).
We validated a technique for quantifying liver fat content based on selective fat and water imaging. The technique is accurate and reproducible and provides a noninvasive method to obtain serial measurements of liver fat content in human subjects.
通过使用肝脏模型校准磁共振成像（MRI）读数，并将人类受试者的MRI测量结果与肝活检标本中肝脂肪含量的估计值进行比较，来验证一种用于测量肝脏脂肪含量的MRI技术。
MRI方案包括使用3.0-T扫描仪通过选择性饱和进行脂肪和水成像。在10名接受肝活检以评估非酒精性脂肪性肝病（NAFLD）的人类受试者中的每一位之前，对一个水体模和肝脏体模进行扫描。人类受试者的肝脏脂肪含量来自通过扫描体模生成的校准曲线。还通过光学图像分析和病理学家对组织学切片的评估来估计肝脏脂肪。
肝脏体模的MRI测量具有高度可重复性。MRI在肝脏两个区域对人类受试者肝脏脂肪含量的测量结果高度相关（r = 0.98，P < 0.001）。MRI测量结果与通过光学图像分析得出的肝脏脂肪含量估计值高度相关（r = 0.96，P < 0.001），与病理学家的估计值也高度相关（r = 0.93，P < 0.001）。
我们验证了一种基于选择性脂肪和水成像来量化肝脏脂肪含量的技术。该技术准确且可重复，提供了一种无创方法来获取人类受试者肝脏脂肪含量的系列测量值。
