Boleti Ana Paula de A, Freire Maria das Graças M, Coelho Mirela B, Silva Walciane da, Baldasso Paulo A, Gomes Valdirene M, Marangoni Sérgio, Novello José C, Macedo Maria Lígia R
Laboratório de Purificação de Proteínas e suas Funções Biológicas, Departamento de Ciências Naturais, Universidade Federal de Mato Grosso do Sul, CP 210, CEP 79603-011 Três Lagoas, MS, Brazil.
J Agric Food Chem. 2007 Apr 4;55(7):2653-8. doi: 10.1021/jf0636317. Epub 2007 Mar 10.
This paper describes the purification and characterization of a novel protein from the seeds of Pouteria torta (family Sapotaceae). The protein was purified by a combination of gel filtration, ion-exchange, and reverse phase chromatographies. SDS-PAGE of the purified protein resulted in a single protein band of 14 kDa in the presence and absence of DTT. The lectin-like activity of pouterin was best inhibited by glycoproteins such as fetuin, asialofetuin, heparin, orosomucoid, and ovoalbumin. Pouterin inhibited the growth of the fungi Fusarium oxysporum and Colletotrichum musae and of the yeast Saccharomyces cerevisiae. The incorporation of pouterin into an artificial diet (final concentration = 0.12%, w/w) caused 50% mortality in larvae of the insect Callosobruchus maculatus, whereas 0.08% pouterin produced an ED50.
本文描述了从桃榄（山榄科）种子中纯化和鉴定一种新型蛋白质的过程。该蛋白质通过凝胶过滤、离子交换和反相色谱法相结合的方法进行纯化。在有和没有二硫苏糖醇（DTT）的情况下，纯化蛋白质的十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）产生了一条14 kDa的单一蛋白带。pouterin的凝集素样活性最好被糖蛋白如胎球蛋白、去唾液酸胎球蛋白、肝素、类粘蛋白和卵清蛋白抑制。pouterin抑制了尖孢镰刀菌、香蕉炭疽菌和酿酒酵母的生长。将pouterin掺入人工饲料（终浓度 = 0.12%，w/w）导致黄斑豆象幼虫50%的死亡率，而0.08%的pouterin产生了半数有效剂量（ED50）。
