Krause Ulf, Harter Christoph, Seckinger Anja, Wolf David, Reinhard Adrian, Bea Florian, Dengler Thomas, Hardt Stefan, Ho Anthony, Katus Hugo A, Kuecherer Helmut, Hansen Alexander
Department of Hematology, University of Heidelberg, Heidelberg, Germany.
Stem Cells Dev. 2007 Feb;16(1):31-7. doi: 10.1089/scd.2006.0089.
Systemic delivery of bone marrow-derived mesenchymal stem cells (MSCs) is a noninvasive approach for myocardial repair. We aimed to test this strategy in a pig model of myocardial infarction. Pigs (n = 8) received autologous MSCs (1 x 10(6)/kg body weight) labeled with fluorescent dye 48 h post proximal left anterior descending artery (LAD) occlusion. Hemodyamics, infarct size, and myocardial function were assessed at baseline and after 1 month. Morphologic analysis revealed that labeled MSCs migrated in the peri-infarct region, resulting in smaller infarct size (32 +/- 7 vs. 19 +/- 7%, p = 0.01), higher fractional area shortening (23 +/- 3 vs. 34.0 +/- 7%, p = 0.001), lower left ventricular end diastolic pressure (18.7 +/- 5 vs. 10.2 +/- 4 mmHg, p = 0.02) and higher +dp/dt (4,570 +/- 540 vs. 6,742 +/- 700 mmHg/s, p = 0.03) during inotropic stimulation. Systemic intravenous delivery of MSCs to pigs limits myocardial infarct size and is an attractive approach for tissue repair.
骨髓间充质干细胞（MSCs）的全身递送是一种用于心肌修复的非侵入性方法。我们旨在在猪心肌梗死模型中测试这一策略。猪（n = 8）在左前降支近端（LAD）闭塞后48小时接受自体MSCs（1×10⁶/千克体重），并用荧光染料标记。在基线和1个月后评估血流动力学、梗死面积和心肌功能。形态学分析显示，标记的MSCs迁移至梗死周边区域，导致梗死面积减小（32±7%对19±7%，p = 0.01），缩短分数增加（23±3%对34.0±7%，p = 0.001），左心室舒张末期压力降低（18.7±5对10.2±4 mmHg，p = 0.02），在变力刺激期间+dp/dt升高（4570±540对6742±700 mmHg/s，p = 0.03）。向猪全身静脉递送MSCs可限制心肌梗死面积，是一种有吸引力的组织修复方法。
