与骨髓干细胞相比，bFGF 和 5-aza-2'-脱氧胞苷预处理后的月经血干细胞在大鼠心肌梗死模型中具有更强的血管生成和免疫调节能力。

Greater angiogenic and immunoregulatory potency of bFGF and 5-aza-2'-deoxycytidine pre-treated menstrual blood stem cells in compare to bone marrow stem cells in rat model of myocardial infarction.

机构信息

Nanobiotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran.

Rajaie Cardiovascular Medical and Research Center, Iran University of Medical Sciences, Tehran, Iran.

出版信息

BMC Cardiovasc Disord. 2022 Dec 31;22(1):578. doi: 10.1186/s12872-022-03032-7.

DOI:10.1186/s12872-022-03032-7

PMID:36587199

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9805241/

Abstract

BACKGROUND

This study is designed to compare the menstrual blood stem cells (MenSCs) and bone marrow stem cells (BMSCs)-secreted factors with or without pre-treatment regimen using basic fibroblast growth factor (bFGF) and 5-aza-2'-deoxycytidine (5-aza) and also regenerative capacity of pre-treated MenSCs and/or BMSCs in a rat model of myocardial infarction (MI).

METHODS

BMSCs and MenSCs were pre-treated with bFGF and 5-aza for 48 h and we compared the paracrine activity by western blotting. Furthermore, MI model was created and the animals were divided into sham, MI, pre-treated BMSCs, and pre-treated MenSCs groups. The stem cells were administrated via tail vain. 35 days post-MI, serum and tissue were harvested for further investigations.

RESULTS

Following pre-treatment, vascular endothelium growth factor, hypoxia-inducible factor-1, stromal cell-derived factor-1, and hepatocyte growth factor were significantly increased in secretome of MenSCs in compared to BMSCs. Moreover, systemic administration of pre-treated MenSCs, leaded to improvement of cardiac function, preservation of myocardium from further subsequent injuries, promotion the angiogenesis, and reduction the level of NF-κB expression in compared to the pre-treated BMSCs. Also, pre-treated MenSCs administration significantly decreased the serum level of Interleukin 1 beta (IL-1β) in compared to the pre-treated BMSCs and MI groups.

CONCLUSIONS

bFGF and 5-aza pre-treated MenSCs offer superior cardioprotection compare to bFGF and 5-aza pre-treated BMSCs following MI.

摘要

背景

本研究旨在比较月经血干细胞（MenSCs）和骨髓间充质干细胞（BMSCs）分泌因子，以及使用碱性成纤维细胞生长因子（bFGF）和 5-氮杂-2'-脱氧胞苷（5-aza）预处理前后的差异，并观察预处理后的 MenSCs 和/或 BMSCs 在大鼠心肌梗死（MI）模型中的再生能力。

方法

用 bFGF 和 5-aza 预处理 BMSCs 和 MenSCs 48 小时，通过 Western blot 比较旁分泌活性。此外，建立 MI 模型，将动物分为假手术组、MI 组、预处理 BMSCs 组和预处理 MenSCs 组。通过尾静脉给予干细胞。MI 后 35 天，采集血清和组织进行进一步研究。

结果

预处理后，MenSCs 分泌的血管内皮生长因子、缺氧诱导因子 1、基质细胞衍生因子 1 和肝细胞生长因子明显高于 BMSCs。此外，与预处理的 BMSCs 相比，系统给予预处理的 MenSCs 可改善心功能，防止心肌进一步损伤，促进血管生成，并降低核因子-κB 表达水平。此外，与预处理的 BMSCs 和 MI 组相比，预处理的 MenSCs 给药可显著降低血清白细胞介素 1β（IL-1β）水平。