Bolcal Cengiz, Yildirim Vedat, Doganci Suat, Sargin Murat, Aydin Ahmet, Eken Ayse, Ozal Ertugrul, Kuralay Erkan, Demirkilic Ufuk, Tatar Harun
Department of Cardiovascular Surgery, Gulhane Military Academy of Medicine, Ankara, Turkey.
J Surg Res. 2007 May 15;139(2):274-9. doi: 10.1016/j.jss.2006.10.043. Epub 2007 Mar 8.
N-acetylcysteine, beta-glucan, and coenzyme Q(10) were shown to have antioxidant and anti-inflammatory effects on reperfusion injury. The aim of our study was to determine and evaluate the effects of these agents on ischemia reperfusion injury of limb.
Forty-four New Zealand white rabbits, all female, weighing between 2.3 to 4.2 (mean 3.8) kg, were used in the study. Four study groups were arranged of 11 animals each, by randomization. The first group was the control group (Group C), the other groups were the Group Q, which was medicated with coenzyme Q10, the Group betaG, which was medicated with beta-glucan, and the Group N, medicated with N-acetylcysteine. After baseline measurements, for the ischemia-reperfusion experiments, common iliac artery was clamped and collateral flow was occluded by a rubber arterial tourniquet wrapped around the thigh at the proximal third of the leg. After 60 min of transient ischemic period, the limb was perfused for 180 min. After perfusion, biopsy was taken from the adductor magnus muscle. Second blood sampling was done after reperfusion period. Blood and tissue analysis were done and evaluated statistically.
Baseline and post-reperfusion levels of glutathione peroxidase (GPx), super oxide dismutase (SOD), malonyldialdehyde (MDA), and nitric oxide (NO) changed significantly. While MDA levels increased in the control group, it decreased in the other study groups. The increase in GPx and SOD levels were significant in all groups except the control group. Levels of NO were found to have decreased in the control group, whereas it had increased in the other groups.
Antioxidant medication may help lowering limb ischemia reperfusion injury. All mentioned medications in our study are shown to be able to have an effective role for preventing ischemia reperfusion injury to some extent through their antioxidant properties.
已表明N-乙酰半胱氨酸、β-葡聚糖和辅酶Q10对再灌注损伤具有抗氧化和抗炎作用。本研究的目的是确定并评估这些药物对肢体缺血再灌注损伤的影响。
本研究使用了44只新西兰白兔，均为雌性，体重在2.3至4.2（平均3.8）千克之间。通过随机分组，将动物分为4个研究组，每组11只。第一组为对照组（C组），其他组分别为用辅酶Q10给药的Q组、用β-葡聚糖给药的βG组和用N-乙酰半胱氨酸给药的N组。在进行基线测量后，进行缺血再灌注实验，通过在大腿近端三分之一处缠绕橡胶动脉止血带，夹闭髂总动脉并阻断侧支血流。在60分钟短暂缺血期后，对肢体进行180分钟的灌注。灌注后，从大收肌取活检组织。在再灌注期结束后进行第二次采血。对血液和组织进行分析并进行统计学评估。
谷胱甘肽过氧化物酶（GPx）、超氧化物歧化酶（SOD）、丙二醛（MDA）和一氧化氮（NO）的基线水平和再灌注后水平发生了显著变化。对照组中MDA水平升高，而其他研究组中MDA水平降低。除对照组外，所有组中GPx和SOD水平的升高均具有显著性。发现对照组中NO水平降低，而其他组中NO水平升高。
抗氧化药物可能有助于减轻肢体缺血再灌注损伤。我们研究中提到的所有药物均显示能够通过其抗氧化特性在一定程度上对预防缺血再灌注损伤发挥有效作用。
