Inal Mine, Altinişik Mehmet, Bilgin Mehmet D
Department of Biochemistry, The Medical School, Osmangazi University, Eskişehir-26480, Turkey.
Cell Biochem Funct. 2002 Dec;20(4):291-6. doi: 10.1002/cbf.953.
Renal ischemia-reperfusion injury occurs in many clinical conditions such as hypovolemic shock, thromboembolism, injury and after renal transplantation. Under these conditions, ROS are considered to be the reason for cellular damage. Bioflavonoids have antioxidant and renoprotective properties. We studied the effect of quercetin, a bioflavonoid, on ischemia and reperfusion in rats. The rats (n = 28) were separated into three groups. Group I was the control group. Animals in groups II (IR) and III (IR + Q) underwent 30 min ischemia and 45 min reperfusion, respectively. Rats, in group III, also received 50 mg kg(-1) quercetin before 45 min of reperfusion. The activities of SOD, CAT, GPx, and concentrations of GSH and GSSGR were determined in renal cortex and erythrocytes. Also, the levels of MDA in renal cortex and plasma, and XO in renal cortex were measured in these groups. The renal cortex XO levels in the IR group were higher than that of the control and IR+Q groups (p<0.001). The renal cortex and plasma MDA levels in the IR group were also found to be higher than the control and IR+Q groups (p<0.01, and p<0.001, respectively). However, a decrease in MAD level of the IR+Q group was found in renal cortex and erythrocytes. In addition, SOD, CAT, and GPx activities in renal cortex and erythrocytes of quercetin-treated animals were enhanced compared to animals of the IR group. Furthermore, there were no significant differences in the SOD, CAT, and GPx activities of the control and IR+Q group. A reduction of GSH and GSSGR levels in IR and IR+Q groups was detected but no significant differences were found between these groups. This study stresses that high concentration of ROS leads to renal ischemia and reperfusion, and quercetin reduces the renal injury by preventing the oxidative stress dependent on ischemia and reperfusion. Quercetin may be used in renal transplantation as an antioxidant drug.
肾缺血再灌注损伤发生于多种临床情况，如低血容量性休克、血栓栓塞、肾损伤以及肾移植术后。在这些情况下，活性氧（ROS）被认为是细胞损伤的原因。生物类黄酮具有抗氧化和肾脏保护特性。我们研究了生物类黄酮槲皮素对大鼠缺血再灌注的影响。将28只大鼠分为三组。第一组为对照组。第二组（IR）和第三组（IR + Q）的动物分别经历30分钟缺血和45分钟再灌注。第三组大鼠在再灌注45分钟前还接受了50 mg·kg⁻¹的槲皮素。测定了肾皮质和红细胞中超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GPx）的活性以及谷胱甘肽（GSH）和氧化型谷胱甘肽（GSSGR）的浓度。此外，还测量了这些组肾皮质和血浆中的丙二醛（MDA）水平以及肾皮质中的黄嘌呤氧化酶（XO）水平。IR组肾皮质XO水平高于对照组和IR + Q组（p < 0.001）。还发现IR组肾皮质和血浆MDA水平高于对照组和IR + Q组（分别为p < 0.01和p < 0.001）。然而，发现IR + Q组肾皮质和红细胞中的MDA水平降低。此外，与IR组动物相比，槲皮素处理动物的肾皮质和红细胞中的SOD、CAT和GPx活性增强。此外，对照组和IR + Q组的SOD、CAT和GPx活性没有显著差异。检测到IR组和IR + Q组中GSH和GSSGR水平降低，但两组之间没有发现显著差异。本研究强调高浓度的ROS导致肾缺血再灌注，而槲皮素通过预防依赖于缺血再灌注的氧化应激来减轻肾损伤。槲皮素可作为抗氧化药物用于肾移植。
