Gao Yuan-Jian, Yan Hong-Li, Ding Fei-Xiang, Lu Yi-Ming, Sun Shu-Han
Department of Medical Genetics, The Second Military Medical University, Xiangyin Road 800, Shanghai 200433, China.
Acta Trop. 2007 Mar;101(3):192-9. doi: 10.1016/j.actatropica.2006.10.014. Epub 2007 Feb 7.
Annexin B1 is a novel member of annexin family firstly cloned by immunological screening a Taenia solium cysticercus library. To investigate the histological distribution and physiological role(s) of this protein, we first prepared a specific monoclonal antibody against annexin B1. Western blot analysis indicated that annexin B1 could be detected in cystic fluid of T. solium cysticercus and sera of pigs/humans with cysticercosis. Thus, annexin B1 might belong to the secreted members of annexins. Immunohistochemical analysis revealed that annexin B1 was mainly present in the tegument of bladders, but not in the scolex and neck; it was also detected in the surrounding host-derived layer with granulomatous infiltration. Together with previous, the presented data suggested that the protein inhibited mammalian PLA2 in vitro, and might down regulate host inflammatory responses.
膜联蛋白B1是膜联蛋白家族的一个新成员，它是通过免疫筛选猪带绦虫囊尾蚴文库首次克隆得到的。为了研究该蛋白的组织学分布和生理作用，我们首先制备了一种针对膜联蛋白B1的特异性单克隆抗体。蛋白质印迹分析表明，在猪带绦虫囊尾蚴的囊液以及患有囊尾蚴病的猪/人的血清中可以检测到膜联蛋白B1。因此，膜联蛋白B1可能属于膜联蛋白的分泌型成员。免疫组织化学分析显示，膜联蛋白B1主要存在于囊尾蚴的皮层中，而在头节和颈部不存在；在有肉芽肿浸润的周围宿主来源层中也检测到了该蛋白。结合之前的研究，目前的数据表明该蛋白在体外可抑制哺乳动物的磷脂酶A2，并且可能下调宿主的炎症反应。
