Obregón-Henao Andrés, Londoño Diana P, Gómez Diana I, Trujillo Judith, Teale Judy M, Restrepo Blanca I
Molecular Parasitology Group, Corporación para Investigaciones Biológicas, Medellín, Colombia.
J Parasitol. 2003 Aug;89(4):726-32. doi: 10.1645/GE-3046.
Taenia solium has a complex life cycle. Its cysticercus can lodge in the brain, causing neurocysticercosis (NCC), and the adult tapeworm's survival in the intestine results in taeniasis. In this study, the in situ detection of previously described glycoprotein antigens used for serological diagnosis of NCC and the detection of other glycoconjugates was explored in cysticerci and the surrounding porcine tissue to understand their potential role in pathogenesis. Immunohistochemistry with an antiserum specific for glycoprotein antigens rich in N-linked carbohydrates and in situ histochemistry with a battery of lectins that have affinity to a variety of glycoconjugates were performed. The glycoconjugates rich in N-linked carbohydrates were detected in the vesicular fluid and tegument of the vesicular membrane and scolex, where the parasite has direct contact with the host tissues during cysticercosis and taeniasis, respectively. Additionally, as the inflammatory response progressed, the parasite's antigenic glycoproteins were also detected in the cytoplasm of inflammatory cells in the surrounding granuloma. In contrast, the spiral canal tegument, which will be exposed to intestinal enzymes in taeniasis, had N-acetyl-galactosamine-rich mucins. Thus, the differential saccharidic composition in T. solium metacestode structures may be important for the survival of the parasite in different host sites.
猪带绦虫具有复杂的生命周期。其囊尾蚴可寄生于脑部，导致神经囊尾蚴病（NCC），而成虫绦虫在肠道内生存会引发猪带绦虫病。在本研究中，对先前用于NCC血清学诊断的糖蛋白抗原进行原位检测，并在囊尾蚴及其周围的猪组织中检测其他糖缀合物，以了解它们在发病机制中的潜在作用。使用对富含N-连接碳水化合物的糖蛋白抗原具有特异性的抗血清进行免疫组织化学，并使用一系列对多种糖缀合物具有亲和力的凝集素进行原位组织化学。在囊泡液、囊泡膜和头节的皮层中检测到富含N-连接碳水化合物的糖缀合物，在囊尾蚴病和猪带绦虫病期间，寄生虫分别在此处与宿主组织直接接触。此外，随着炎症反应的进展，在周围肉芽肿的炎症细胞胞质中也检测到寄生虫的抗原性糖蛋白。相比之下，在猪带绦虫病中会暴露于肠道酶的螺旋管皮层含有富含N-乙酰半乳糖胺 的粘蛋白。因此，猪带绦虫中绦期结构的不同糖组成可能对寄生虫在不同宿主部位的生存很重要。
