van der Merwe Johannes A, Dubery Ian A
Department of Biochemistry, University of Johannesburg, Kingsway Campus, Aucklandpark, South Africa.
J Plant Physiol. 2007 Sep;164(9):1231-4. doi: 10.1016/j.jplph.2006.11.009. Epub 2007 Mar 9.
A cDNA, up-regulated upon treatment of tobacco cells with salicylic acid and benzo (1,2,3) thiadiazole-7-carbothioic acid S-methyl ester, was identified by differential RNA display and the full sequence obtained. This mitochondrial gene, twin arginine translocation (tatC), resembles orthologues across different species, including the gene that codes for a sec-independent membrane translocating protein in bacteria. Hypothetical tatC proteins have also been identified in the mitochondria of Arabidopsis thaliana, Oenothera berteriana, Beta vulgaris, Oryza sativa and Marchantia polymorpha. Comparative protein analysis indicates a similar function for the tatC gene. The up-regulation of the tatC gene in a 3kbp transcript was confirmed by RNA gel blot analysis.
通过差异RNA显示技术鉴定出一个在水杨酸和苯并（1,2,3）噻二唑-7-硫代羧酸S-甲酯处理烟草细胞后上调的cDNA，并获得了其完整序列。这个线粒体基因，双精氨酸转运（tatC），类似于不同物种中的直系同源物，包括编码细菌中一种不依赖sec的膜转运蛋白的基因。在拟南芥、月见草、甜菜、水稻和地钱的线粒体中也鉴定出了假定的tatC蛋白。比较蛋白质分析表明tatC基因具有相似的功能。通过RNA凝胶印迹分析证实了tatC基因在一个3kbp转录本中的上调。
