Plant-Microbe Interactions, Institute of Environmental Biology, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands.
Mol Plant Microbe Interact. 2011 Apr;24(4):395-407. doi: 10.1094/MPMI-05-10-0115.
Systemically induced resistance is a promising strategy to control plant diseases, as it affects numerous pathogens. However, since induced resistance reduces one or both growth and activity of plant pathogens, the indigenous microflora may also be affected by an enhanced defensive state of the plant. The aim of this study was to elucidate how much the bacterial rhizosphere microflora of Arabidopsis is affected by induced systemic resistance (ISR) or systemic acquired resistance (SAR). Therefore, the bacterial microflora of wild-type plants and plants affected in their defense signaling was compared. Additionally, ISR was induced by application of methyl jasmonate and SAR by treatment with salicylic acid or benzothiadiazole. As a comparative model, we also used wild type and ethylene-insensitive tobacco. Some of the Arabidopsis genotypes affected in defense signaling showed altered numbers of culturable bacteria in their rhizospheres; however, effects were dependent on soil type. Effects of plant genotype on rhizosphere bacterial community structure could not be related to plant defense because chemical activation of ISR or SAR had no significant effects on density and structure of the rhizosphere bacterial community. These findings support the notion that control of plant diseases by elicitation of systemic resistance will not significantly affect the resident soil bacterial microflora.
系统诱导抗性是控制植物病害的一种很有前途的策略，因为它可以影响许多病原体。然而，由于诱导抗性降低了植物病原体的一种或两种生长和活性，植物的土著微生物群落也可能受到植物增强的防御状态的影响。本研究的目的是阐明拟南芥的根际细菌微生物群落受系统诱导抗性（ISR）或系统获得性抗性（SAR）影响的程度。因此，比较了野生型植物和防御信号受到影响的植物的细菌根际微生物群落。此外，通过施用茉莉酸甲酯诱导 ISR，通过水杨酸或噻苯隆处理诱导 SAR。作为比较模型，我们还使用了野生型和乙烯不敏感的烟草。一些防御信号受到影响的拟南芥基因型在其根际中的可培养细菌数量发生了变化；然而，这些影响取决于土壤类型。植物基因型对根际细菌群落结构的影响不能与植物防御相关联，因为 ISR 或 SAR 的化学激活对根际细菌群落的密度和结构没有显著影响。这些发现支持这样一种观点，即通过激发系统抗性来控制植物病害不会显著影响驻留土壤细菌微生物群落。
