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用于单核细胞增生李斯特菌的改良荧光素酶标记系统可实现体内和体外实时监测。

Improved luciferase tagging system for Listeria monocytogenes allows real-time monitoring in vivo and in vitro.

作者信息

Riedel Christian U, Monk Ian R, Casey Pat G, Morrissey David, O'Sullivan Gerald C, Tangney Mark, Hill Colin, Gahan Cormac G M

机构信息

Alimentary Pharmabiotic Centre and Department of Microbiology, University College Cork, Cork, Ireland.

出版信息

Appl Environ Microbiol. 2007 May;73(9):3091-4. doi: 10.1128/AEM.02940-06. Epub 2007 Mar 9.

DOI:10.1128/AEM.02940-06
PMID:17351089
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1892880/
Abstract

An improved system for luciferase tagging Listeria monocytogenes was developed by constructing a highly active, constitutive promoter. This construct gave 100-fold-higher activity in broth than any native promoter tested and allowed for imaging of lux-tagged L. monocytogenes in food products, during murine infections, and in tumor targeting studies.

摘要

通过构建一个高活性的组成型启动子，开发出了一种改进的用于荧光素酶标记单核细胞增生李斯特菌的系统。该构建体在肉汤中的活性比所测试的任何天然启动子高100倍，并能够对食品中、小鼠感染期间以及肿瘤靶向研究中的荧光素酶标记的单核细胞增生李斯特菌进行成像。

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Cancer Gene Ther. 2006 Dec;13(12):1061-71. doi: 10.1038/sj.cgt.7700976. Epub 2006 Jul 28.
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An updated review of Listeria monocytogenes in the pork meat industry and its products.猪肉行业及其产品中单核细胞增生李斯特菌的最新综述。
J Appl Microbiol. 2006 Jul;101(1):7-17. doi: 10.1111/j.1365-2672.2006.02962.x.
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Novel luciferase reporter system for in vitro and organ-specific monitoring of differential gene expression in Listeria monocytogenes.用于体外和器官特异性监测单核细胞增生李斯特菌中差异基因表达的新型荧光素酶报告系统。
Appl Environ Microbiol. 2006 Apr;72(4):2876-84. doi: 10.1128/AEM.72.4.2876-2884.2006.
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Bacterial gene therapy strategies.细菌基因治疗策略。
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Citrobacter rodentium of mice and man.小鼠和人类的鼠柠檬酸杆菌
Cell Microbiol. 2005 Dec;7(12):1697-706. doi: 10.1111/j.1462-5822.2005.00625.x.
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Mol Microbiol. 2005 Sep;57(5):1460-73. doi: 10.1111/j.1365-2958.2005.04780.x.
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Monitoring age-related susceptibility of young mice to oral Salmonella enterica serovar Typhimurium infection using an in vivo murine model.使用体内小鼠模型监测幼鼠对口服肠炎沙门氏菌鼠伤寒血清型感染的年龄相关易感性。
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