Institute of Microbiology and Biotechnology, University of Ulm, Ulm, Germany.
Microbiologyopen. 2022 Aug;11(4):e1304. doi: 10.1002/mbo3.1304.
The global increase in antibiotic resistance of pathogenic microorganisms requires the identification and characterization of novel antimicrobials. Bacterial biosensors expressing fluorescent proteins such as pHluorin variants are suitable for high-throughput screenings. Here, we present Listeria spp. pH-sensitive biosensors with improved fluorescence for single-cell analysis of antimicrobials by flow cytometry.
全球范围内致病微生物对抗生素的耐药性不断增加，这就需要我们寻找并鉴定新型的抗菌药物。表达荧光蛋白（如 pHlourin 变体）的细菌生物传感器非常适合高通量筛选。本研究构建了具有改良荧光性能的李斯特氏菌 pH 敏感型生物传感器，用于通过流式细胞术对单个细胞进行药敏分析。
