Anderson P A, Moosler A, Grimmelikhuijzen C J
Center for Molecular Neurobiology, University of Hamburg, Federal Republic of Germany.
Cell Tissue Res. 1992 Jan;267(1):67-74. doi: 10.1007/BF00318692.
The nervous systems of the scyphomedusae Chrysaora hysoscella, Cyanea capillata and Cyanea lamarckii (Phylum Cnidaria) were stained using an anti-serum against the anthozoan neuropeptide Antho-RFamide. Staining was widespread in all three species. In Chrysaora, the antiserum revealed nerve nets in the subumbrella and exumbrella ectoderm, in both faces of the oral lobes, and in the endoderm lining the subumbrella and exumbrella surfaces of the gastric cavity. The most prominent staining occurred in a dense plexus of neurons in the ectoderm at the base of the tentacles. This nerve net sent projections into the subumbrella ectoderm. For the most part, staining in the two species of Cyanea was similar to that in Chrysaora, with a few exceptions. These include the presence, in Cyanea, of an obvious tentacular nerve tract and nerve nets associated with clusters of cnidocytes in the tentacles. Radioimmunoassays of extracts from Chrysaora and Cyanea lamarkii revealed that both species contain large amounts of Antho-RFamide-like material (up to 55 nmol/animal). The results indicate that Antho-RFamide-like neuropeptides are widespread in scyphomedusae.
利用抗珊瑚虫神经肽Antho - RFamide的抗血清对钵水母纲的金黄水母、北极霞水母和拉氏霞水母（刺胞动物门）的神经系统进行染色。在这三个物种中染色都很普遍。在金黄水母中，抗血清显示在伞下和伞外的外胚层、口叶的两面以及胃腔伞下和伞外表面的内胚层中有神经网。最显著的染色出现在触手基部外胚层的密集神经元丛中。这个神经网向伞下外胚层发出投射。在大多数情况下，两种霞水母的染色与金黄水母相似，但有一些例外。这些例外包括在霞水母中存在明显的触手神经束以及与触手中刺细胞簇相关的神经网。对金黄水母和拉氏霞水母提取物的放射免疫分析表明，这两个物种都含有大量的类Antho - RFamide物质（高达55纳摩尔/动物）。结果表明，类Antho - RFamide神经肽在钵水母中广泛存在。
