Grimmelikhuijzen C J, Graff D
Proc Natl Acad Sci U S A. 1986 Dec;83(24):9817-21. doi: 10.1073/pnas.83.24.9817.
A radioimmunoassay has been developed for peptides containing the carboxyl-terminal sequence Arg-Phe-NH2 (RFamide). Using this radioimmunoassay and applying cation-exchange chromatography and HPLC, we have isolated an RFamide peptide from acetic acid extracts of the sea anemone Anthopleura elegantissima. Three different methods established that the structure of the Anthopleura RFamide peptide (Antho-RFamide) is pyroGlu-Gly-Arg-Phe-NH2. Comparison of synthetic and natural Antho-RFamide and their enzymatic breakdown products on six different HPLC columns confirmed the structure of the sea anemone peptide. Using synthetic Antho-RFamide as a standard in our radioimmunoassay, we measured high concentrations (3.2 nmol/g wet weight) of this peptide in extracts of Anthopleura. It is proposed that Antho-RFamide is a transmitter at neuromuscular synapses in sea anemones.
已开发出一种针对含有羧基末端序列精氨酸 - 苯丙氨酸 - 氨基（RFamide）的肽的放射免疫测定法。利用这种放射免疫测定法，并应用阳离子交换色谱法和高效液相色谱法，我们从海葵华丽列指海葵的乙酸提取物中分离出了一种RFamide肽。三种不同的方法确定了华丽列指海葵RFamide肽（Antho - RFamide）的结构为焦谷氨酸 - 甘氨酸 - 精氨酸 - 苯丙氨酸 - 氨基。在六种不同的高效液相色谱柱上对合成的和天然的Antho - RFamide及其酶解产物进行比较，证实了海葵肽的结构。在我们的放射免疫测定中使用合成的Antho - RFamide作为标准品，我们测量了华丽列指海葵提取物中该肽的高浓度（3.2纳摩尔/克湿重）。有人提出Antho - RFamide是海葵神经肌肉突触处的一种递质。
